Počet záznamů: 1
Seed development, seed germination and seedling growth in the R50 (sym16) pea mutant are not directly linked to altered cytokinin homeostasis
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SYSNO ASEP 0382517 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Seed development, seed germination and seedling growth in the R50 (sym16) pea mutant are not directly linked to altered cytokinin homeostasis Tvůrce(i) Long, C. L. (CA)
Held, M. (CA)
Hayward, A. (CA)
Nisler, Jaroslav (UEB-Q) RID, ORCID
Spíchal, Lukáš (UEB-Q) RID, ORCID
Emery, R. J. N. (CA)
Moffatt, B. A. (CA)
Guinel, F. C. (CA)Zdroj.dok. Physiologia Plantarum. - : Wiley - ISSN 0031-9317
Roč. 145, č. 2 (2012), s. 341-359Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. DK - Dánsko Klíč. slova PISUM-SATIVUM-L ; ARABIDOPSIS-THALIANA ; NODULATION MUTANT Vědní obor RIV ED - Fyziologie CEP LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000303798500008 DOI 10.1111/j.1399-3054.2012.01594.x Anotace R50 (sym16) is a pea nodulation mutant that accumulates cytokinin (CK) in its vegetative organs. Total CK content increases as the plant ages because of the low activity of the enzyme cytokinin oxidase/dehydrogenase (CKX) responsible for CK degradation. R50 exhibits a large seed with high relative water content, and its seedling establishes itself slowly. Whether these two traits are linked to abnormal CK levels was considered here. R50 was found to have a similar germination rate but a much slower epicotyl emergence than Sparkle, its wild-type (WT). At the onset of emergence, the starch grains in R50 cotyledons were larger than those of WT; furthermore, they did not degrade as fast as in WT because of low amylase activity. No differences between the pea lines were observed in the CK forms identified during seed embryogenesis. However, while CK content compared to that of WT was reduced early in R50 embryogenesis, it was elevated later on in its dry seeds where CKX activity was low, although CKX transcript abundance remained high. Transcripts of the two known PsCKX isoforms exhibited tissue- and development-specific profiles with no detectable PsCKX2 expression in cotyledons. There were more of both transcripts in R50 roots than in WT roots, but less of PsCKX2 than PsCKX1 in R50 shoots compared to WT shoots. Thus, although there is a definite CKX post-transcriptional defect in R50 dry seeds, an abnormal CK homeostasis is not the basis of the delay in R50 seedling establishment, which we linked to abnormal amylase activity early in development. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1