Počet záznamů: 1
Cytokinin Regulation of Gene Expression in the AHP Gene Family in Arabidopsis thaliana
- 1.
SYSNO ASEP 0305973 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Cytokinin Regulation of Gene Expression in the AHP Gene Family in Arabidopsis thaliana Překlad názvu Cytokinin Regulation of Gene Expression in the AHP Gene Family in Arabidopsis thaliana Tvůrce(i) Hradilová, Jana (BFU-R)
Malbeck, Jiří (UEB-Q) RID, ORCID
Brzobohatý, Břetislav (BFU-R) RID, ORCIDZdroj.dok. Journal of Plant Growth Regulation. - : Springer - ISSN 0721-7595
Roč. 26, č. 3 (2007), s. 229-244Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova gene expression ; AHP gene family ; cytokinin signal transduction Vědní obor RIV EF - Botanika CEP LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 1M06030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IAA600380507 GA AV ČR - Akademie věd IAA600040612 GA AV ČR - Akademie věd Další zdroj jiné veřejné zdrojejiné veřejné zdrojejiné veřejné zdrojejiné veřejné zdroje CEZ AV0Z50380511 - UEB-Q (2005-2011) AV0Z50040702 - BFU-R (2007-2013) DOI 10.1007/s00344-007-9011-x Anotace In higher plants histidine-aspartate phosphorelays are involved in hormone and stress signaling via a two-component system of signal transduction. In this system a histidine-containing phosphotransmitter (HPt) mediates signal transmission from a sensory histidine kinase to a response regulator, providing integration and/or branching of several different signaling pathways. Five genes encoding HPts, AHP1-5, have been identified in Arabidopsis. Histidine-aspartate phosphorelays involving HPts have been at least partly implicated in cytokinin signaling. We analyzed the regulation by cytokinins of AHP gene expression. We compared the effects on steady-state levels of AHP transcripts of a short-term treatment with an aromatic cytokinin and increase in endogenous isoprenoid cytokinin levels using an activable ipt system in 8-day-old Arabidopsis seedlings. Following ipt activation, a rapid and highly preferential increase in trans-zeatin-type cytokinins was observed, whereas other isoprenoid-type cytokinins showed no or only marginal increases. The levels of cytokinin metabolites under long-term ipt activation suggest that the seedlings may have difficulties in efficiently downregulating active forms of the hormone. Using real-time RT-PCR, transient increases in steady-state levels of AHP1-4 transcripts in response to both the short-term N-6-benzyladenine treatment and the increase in endogenous trans-zeatin-type cytokinin levels were observed. In contrast, both the full and the alternatively spliced AHP5 transcripts remained unaltered. On the other hand, increases in steady-state levels of AHP1-4 transcripts observed in seedlings cultivated continuously in the presence of exogenous N-6-benzyladenine were not paralleled in seedlings with constitutively increased endogenous trans-zeatin-type cytokinins, providing further indirect evidence for distinct functions of aromatic and isoprenoid cytokinins. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2008
Počet záznamů: 1