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Proteomics of CDK inhibition in cancer cells
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SYSNO ASEP 0090507 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Stať ve sborníku Název Proteomics of CDK inhibition in cancer cells Překlad názvu Proteomika CDK inhibice v nádorových buňkách Tvůrce(i) Kovářová, Hana (UZFG-Y) RID, ORCID
Skalníková, Helena (UZFG-Y) RID, ORCID
Halada, Petr (MBU-M) RID, ORCID
Strnad, M. (CZ)
Hajdúch, M. (CZ)Zdroj.dok. 3rd Symposium and Workshop on Molecular Pathology . - Olomouc : -, 2007
S. 1-1Poč.str. 1 s. Akce Symposium and Workshop on Molecular Pathology /3./ Datum konání 04.05.2007-05.05.2007 Místo konání Olomouc Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova cyclin-dependent kinase inhibitors ; cancer ; proteomics Vědní obor RIV EB - Genetika a molekulární biologie CEP GA301/05/0418 GA ČR - Grantová agentura ČR LC07017 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50450515 - UZFG-Y (2005-2011) AV0Z50200510 - MBU-M (2005-2011) Anotace In order to improve our understanding of the biochemical basis of the anti-cancer activity of olomoucine-derived synthetic cyclin-dependent kinase inhibitors (CDKIs) and to search for novel proteins associated with these biological effects we applied complex proteomic approaches. To analyse cellular responses to the CDKI we used two cancer models: the CEM T-lymphoblastic leukemia cell line representing hematological malignancy, and the A549 lung adenocarcinoma cell line as a solid tumor model. Cancer cells of these lines were cultured in both the presence and absence (controls) of the CDKI, bohemine (BOH). Cellular proteins of both of these lines were then extracted and fractionated using conventional two-dimensional gel electrophoresis (2-DE), and for the CEM T-lymphoblastic leukemia cell line we also used a 2-D liquid phase fractionation system ProteomeLab PF 2D ( Beckman Coulter). Computer-assisted data analysis of the resulting 2-D protein expression maps was applied to determine the similarity/dissimilarity of the maps and to select characteristic protein spots or bands based on the quantitative differences between BOH-treated and control cells. Many of these differentially expressed proteins were identified by mass spectrometry, since they represent candidate biomarkers of cancer cell responses to CDK inhibition and cellular pathways that are relevant to the anti-cancer activity of the CDKIs. Subsequently, we focused directly on these proteins in confirmatory studies using various techniques (including quantitative immunoblotting, immunocytochemistry and functional activity analyses) to demonstrate the validity of the proteomic results and extend our knowledge of the CDKI effects. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2008
Počet záznamů: 1