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Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization
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SYSNO ASEP 0585159 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization Tvůrce(i) Kindermann, Marek (UOCHB-X)
Neburková, Jitka (UOCHB-X) RID, ORCID
Neuhöferová, Eva (MBU-M)
Majer, Jan (UOCHB-X)
Štejfová, Miroslava (UOCHB-X) ORCID
Benson, Veronika (MBU-M) RID, ORCID
Cígler, Petr (UOCHB-X) RID, ORCIDČíslo článku 2314088 Zdroj.dok. Advanced Functional Materials - ISSN 1616-301X
(2024)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova cationic copolymer ; nanodiamond ; siRNA delivery ; transfection CEP EH22_008/0004558 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA23-04876S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 ; MBU-M - RVO:61388971 DOI 10.1002/adfm.202314088 Anotace The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval of several small interfering RNA (siRNA)-based drugs. However, the efficacy of siRNA delivery is still limited, and an extensive search for alternative and highly effective delivery approaches is ongoing. With this aim, three generations of non-viral vectors based on modified nanodiamonds (NDs) have been gradually developed in the past decade. They show great promise due to the negligible toxicity of the ND core. Here, a robust methodological approach is presented to enable the evaluation of new vector nanomaterials. Using a new type of third-generation ND vector coated with a copolymer with tunable charge density, variables such as colloidal stability, surface electrostatic properties, the molecular composition of the copolymer, and the mode of complexation with siRNA are optimized. Using an innovative data processing strategy, the results are related to biological potency, toxicity, and cell proliferation. Finally, the optimized composition of a coating copolymer consisting of a cationic component, 2-dimethylaminoethyl methacrylate, and an electroneutral biocompatible component, N-(2-hydroxypropyl) methacrylamide, is evaluated. The optimized NDs vectors are colloidally and biologically stable siRNA delivery tools with broad potential for RNA interference-based therapeutics. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2025 Elektronická adresa https://doi.org/10.1002/adfm.202314088
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