Počet záznamů: 1
The disruption of circadian rhythmicity of gene expression in the hippocampus and associated structures in Gria2R/R mice, a comparison with C57BL/6J and Adar2−/− mice strains
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SYSNO ASEP 0583680 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The disruption of circadian rhythmicity of gene expression in the hippocampus and associated structures in Gria2R/R mice, a comparison with C57BL/6J and Adar2−/− mice strains Tvůrce(i) Lebedeva, M. (CZ)
Kubištová, A. (CZ)
Spišská, V. (CZ)
Filipovská, E. (CZ)
Pačesová, D. (CZ)
Svobodová, I. (CZ)
Kuchtiak, Viktor (FGU-C) ORCID
Balík, Aleš (FGU-C) RID, ORCID
Bendová, Z. (CZ)Číslo článku 148739 Zdroj.dok. Brain Research. - : Elsevier - ISSN 0006-8993
Roč. 1826, 1 March (2024)Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova circadian rhythms ; Adar2 ; Gria2 ; mice ; hippocampus ; suprachiasmatic nucleus Obor OECD Neurosciences (including psychophysiology CEP EF16_025/0007444 GA MZd - Ministerstvo zdravotnictví Institucionální podpora FGU-C - RVO:67985823 UT WOS 001165646600001 EID SCOPUS 85181063268 DOI 10.1016/j.brainres.2023.148739 Anotace Adar2-/- mice are a widely used model for studying the physiological consequences of reduced RNA editing. These mice are viable only when the Q/R editing site of the Gria2 subunit of the AMPA receptor is constitutively mutated to the codon for arginine, and Gria2R/R mice often serve as the sole control for Adar2-/- mice. Our study aimed to investigate whether ADAR2 inactivity and the Gria2R/R phenotype affect the rhythmicity of the circadian clock gene pattern and the expression of Gria1 and Gria2 subunits in the suprachiasmatic nucleus (SCN), hippocampus, parietal cortex and liver. Our data show that Gria2R/R mice completely lost circadian rhythmicity in the hippocampus compared to Adar2-/- mice. Compared to C57BL/6J mice, the expression profiles in the hippocampus and parietal cortex of Gria2R/R mice differ to the same extent as in Adar2-/-. No alterations were detected in the circadian profiles in the livers. These data suggest that the natural gradual postnatal increase in the editing of the Q/R site of the Gria2 subunit may be important for the development of circadian clockwork in some brain structures, and the use of Gria2R/R mice as the only control to Adar2-/- mice in the experiments dependent on the hippocampus and parietal cortex should therefore be considered. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2025 Elektronická adresa https://doi.org/10.1016/j.brainres.2023.148739
Počet záznamů: 1