Počet záznamů: 1
Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH receptor activation in GH1 cells
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SYSNO ASEP 0580215 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH receptor activation in GH1 cells Tvůrce(i) Drastichová, Z. (CZ)
Trubačová, Radka (FGU-C)
Novotný, J. (CZ)Číslo článku 115830 Zdroj.dok. Biomedicine & Pharmacotherapy. - : Elsevier - ISSN 0753-3322
Roč. 168, Dec (2023)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova β-arrestin2 ; cell cycle ; phosphosignaling ; Taltirelin ; Thyrotropin-releasing hormone Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 001113579600001 EID SCOPUS 85175557838 DOI 10.1016/j.biopha.2023.115830 Anotace Thyrotropin-releasing hormone (TRH) is known to activate several cellular signaling pathway, but the activation of the TRH receptor (TRH-R) has not been reported to regulate gene transcription. The aim of this study was to identify phosphosignaling pathways and phosphoprotein complexes associated with gene transcription in GH1 pituitary cells treated with TRH or its analog, taltirelin (TAL), using label-free bottom-up mass spectrometry-based proteomics. Our detailed analysis provided insight into the mechanism through which TRH-R activation may regulate the transcription of genes related to the cell cycle and proliferation. It involves control of the signaling pathways for β-catenin/Tcf, Notch/RBPJ, p53/p21/Rbl2/E2F, Myc, and YY1/Rb1/E2F through phosphorylation and dephosphorylation of their key components. In many instances, the phosphorylation patterns of differentially phosphorylated phosphoproteins in TRH- or TAL-treated cells were identical or displayed a similar trend in phosphorylation. However, some phosphoproteins, especially components of the Wnt/β-catenin/Tcf and YY1/Rb1/E2F pathways, exhibited different phosphorylation patterns in TRH- and TAL-treated cells. This supports the notion that TRH and TAL may act, at least in part, as biased agonists. Additionally, the deficiency of β-arrestin2 resulted in a reduced number of alterations in phosphorylation, highlighting the critical role of β-arrestin2 in the signal transduction from TRH-R in the plasma membrane to transcription factors in the nucleus. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2024 Elektronická adresa https://doi.org/10.1016/j.biopha.2023.115830
Počet záznamů: 1