Počet záznamů: 1  

T3SS chaperone of the CesT family is required for secretion of the anti-sigma factor BtrA in Bordetella pertussis

  1. 1.
    SYSNO ASEP0578519
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevT3SS chaperone of the CesT family is required for secretion of the anti-sigma factor BtrA in Bordetella pertussis
    Tvůrce(i) Držmíšek, Jakub (MBU-M) ORCID
    Petráčková, Denisa (MBU-M) RID, ORCID
    Dienstbier, Ana (MBU-M) ORCID
    Čurnová, Ivana (MBU-M) ORCID
    Večerek, Branislav (MBU-M) RID, ORCID
    Číslo článku2272638
    Zdroj.dok.Emerging Microbes & Infections . - : Nature Publishing Group
    Roč. 12, č. 2 (2023)
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaenteropathogenic escherichia-coli ; iii secretion ; virulence ; system ; bronchiseptica ; locus ; pathogenesis ; adaptation ; motility ; provides ; Bordetella pertussis ; t3ss ; CesT chaperone ; anti-sigma factor ; biofilm
    Obor OECDMicrobiology
    CEPGA23-05634S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS001095744600001
    EID SCOPUS85175561930
    DOI10.1080/22221751.2023.2272638
    AnotaceBordetella pertussis is a Gram-negative, strictly human re-emerging respiratory pathogen and the causative agent of whooping cough. Similar to other Gram-negative pathogens, B. pertussis produces the type III secretion system, but its role in the pathogenesis of B. pertussis is enigmatic and yet to be elucidated. Here, we combined RNA-seq, LC-MS/MS, and co-immunoprecipitation techniques to identify and characterize the novel CesT family T3SS chaperone BP2265. We show that this chaperone specifically interacts with the secreted T3SS regulator BtrA and represents the first non-flagellar chaperone required for the secretion of an anti-sigma factor. In its absence, secretion but not production of BtrA and most T3SS substrates is severely impaired. It appears that the role of BtrA in regulating T3SS extends beyond its activity as an antagonist of the sigma factor BtrS. Predictions made by artificial intelligence system AlphaFold support the chaperone function of BP2265 towards BtrA and outline the structural basis for the interaction of BtrA with its target BtrS. We propose to rename BP2265 to BtcB for the Bordetella type III chaperone of BtrA.In addition, the absence of the BtcB chaperone results in increased expression of numerous flagellar genes and several virulence genes. While increased production of flagellar proteins and intimin BipA translated into increased biofilm formation by the mutant, enhanced production of virulence factors resulted in increased cytotoxicity towards human macrophages. We hypothesize that these phenotypic traits result indirectly from impaired secretion of BtrA and altered activity of the BtrA/BtrS regulatory node.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2024
    Elektronická adresahttps://www.tandfonline.com/doi/full/10.1080/22221751.2023.2272638
Počet záznamů: 1  

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