Počet záznamů: 1
Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models
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SYSNO ASEP 0571902 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models Tvůrce(i) Kevély, Á. (SK)
Prančlová, Veronika (BC-A) ORCID
Slávikova, M. (SK)
Haviernik, Jan (BC-A) RID, ORCID
Hönig, Václav (BC-A) RID, ORCID
Nováková, E. (SK)
Palus, Martin (BC-A) RID, ORCID
Růžek, Daniel (BC-A) RID, ORCID
Klempa, B. (SK)
Kočí, J. (SK)Celkový počet autorů 10 Číslo článku 2673 Zdroj.dok. Viruses. - : MDPI
Roč. 14, č. 12 (2022)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova tick-borne encephalitis virus ; tbev ; mCherry reporter ; viral reverse genetics ; Ixodes ricinus ; ticks ; tick cell culture Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Virology CEP GA20-14325S GA ČR - Grantová agentura ČR GA20-30500S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000904310800001 EID SCOPUS 85144505223 DOI 10.3390/v14122673 Anotace The tick-borne encephalitis virus (TBEV) causes a most important viral life-threatening illness transmitted by ticks. The interactions between the virus and ticks are largely unexplored, indicating a lack of experimental tools and systematic studies. One such tool is recombinant reporter TBEV, offering antibody-free visualization to facilitate studies of transmission and interactions between a tick vector and a virus. In this paper, we utilized a recently developed recombinant TBEV expressing the reporter gene mCherry to study its fitness in various tick-derived in vitro cell cultures and live unfed nymphal Ixodes ricinus ticks. The reporter virus was successfully replicated in tick cell lines and live ticks as confirmed by the plaque assay and the mCherry-specific polymerase chain reaction (PCR). Although a strong mCherry signal determined by fluorescence microscopy was detected in several tick cell lines, the fluorescence of the reporter was not observed in the live ticks, corroborated also by immunoblotting. Our data indicate that the mCherry reporter TBEV might be an excellent tool for studying TBEV-tick interactions using a tick in vitro model. However, physiological attributes of a live tick, likely contributing to the inactivity of the reporter, warrant further development of reporter-tagged viruses to study TBEV in ticks in vivo. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1999-4915/14/12/2673
Počet záznamů: 1