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QUANTITATIVE ANALYSIS OF SYNCYTIN-1 BINDING TO ITS RECEPTOR
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SYSNO ASEP 0567915 Druh ASEP A - Abstrakt Zařazení RIV O - Ostatní Název QUANTITATIVE ANALYSIS OF SYNCYTIN-1 BINDING TO ITS RECEPTOR Tvůrce(i) Štafl, Kryštof (UMG-J)
Trávníček, Martin (UMG-J)
Hejnar, Jiří (UMG-J) RID
Trejbalová, Kateřina (UMG-J) RIDČíslo článku P-62 Zdroj.dok. Czech Chemical Society Symposium Series - ISSN 2336-7202
Roč. 20, č. 6 (2022)Poč.str. 1 s. Akce Annual meeting of the National Institute of Virology and Bacteriology (NIVB) /1./ Datum konání 30.11.2022 - 02.12.2022 Místo konání Kutná Hora Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova syncytin-1 ; ASCT2 ; endogenous retrovirus Obor OECD Virology CEP LX22NPO5103 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 Anotace Syncytin-1 is a fusogenic glycoprotein of retroviral origin that is specifically expressed in the human placenta. Syncytin-1 induces the fusion of cellular membranes of cytotrophoblasts after interaction with its cellular receptor, ASCT2. This process results in the formation of a multi-nuclear syncytiotrophoblast layer, which facilitates the transport of nutrients and metabolites between the mother and the fetus and is required for a successful pregnancy. Mutations in Syncytin-1 or ASCT2 can impair their interaction which may lead to disorders like eclampsia, recurrent pregnancy loss or idiopathic infertility. Up to now, aproper description of the Syncytin-1-ASCT2 binding is missing.We developed a multi-level approach to functionally analyze the efficiency of the Syncytin-1-ASCT2 interaction in a cell system. Ectopically expressed variants of ASCT2 are combined with fluorescent and luminescent proteins and can be instantly detected by microscopy, flow cytometry and common plate readers. We can routinely check for the receptor expression on mRNA and protein levels and validate its localization. We also prepared tools employing Syncytin-1: specific immunoadhesin, infectious virus and reporter cells detecting cellular fusion. With these tools, we can assess ASCT2 ability to bind and prime Syncytin-1.Our system can facilitate precise characterization of the Syncytin-1 binding site on the receptor and lead to detailed molecular understanding of one of the critical steps in human placenta morphogenesis. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa http://www.ccsss.cz/index.php/ccsss/issue/view/37/67
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