Počet záznamů: 1
Cytokinin oxidase/dehydrogenase inhibitors: outlook for selectivity and high efficiency
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SYSNO ASEP 0562506 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytokinin oxidase/dehydrogenase inhibitors: outlook for selectivity and high efficiency Tvůrce(i) Nisler, Jaroslav (UEB-Q) RID, ORCID
Pěkná, Z. (CZ)
Končitíková, R. (CZ)
Klimeš, P. (CZ)
Kadlecová, A. (CZ)
Murvanidze, N. (BE)
Werbrouck, S. (BE)
Plačková, Lenka (UEB-Q) ORCID, RID
Kopečný, D. (CZ)
Zalabák, David (UEB-Q) ORCID
Spíchal, L. (CZ)
Strnad, Miroslav (UEB-Q) RID, ORCIDCelkový počet autorů 12 Zdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 73, č. 14 (2022), s. 4806-4817Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Agriculture ; biotechnology ; Caenorhabditis elegans ; CKX inhibitor ; cytokinin ; cytokinin oxidase/dehydrogenase ; diphenylurea ; nematodes ; plant hormones ; plant tissue culture Obor OECD Biochemistry and molecular biology CEP GA21-07661S GA ČR - Grantová agentura ČR TP01010037 GA TA ČR - Technologická agentura ČR EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000808214600001 EID SCOPUS 85137382315 DOI 10.1093/jxb/erac201 Anotace Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation of cytokinins in plants, and this effect can be exploited in agriculture and in plant tissue culture. In this study, we examine the structure–activity relationship of two series of CKX inhibitors based on diphenylurea. The compounds of Series I were derived from the recently published CKX inhibitors 3TFM-2HM and 3TFM-2HE, and we identified key substituents with increased selectivity for maize ZmCKX1 and ZmCKX4a over AtCKX2 from Arabidopsis. Series II contained compounds that further exceled in CKX inhibitory activity as well as in the ease of their synthesis. The best inhibitors exhibited half-maximal inhibitory concentration (IC50) values in low nanomolar ranges with ZmCKX1 and especially with ZmCKX4a, which is generally more resistant to inhibition. The activity of the key compounds was verified in tobacco and lobelia leaf-disk assays, where N6-isopentenyladenine was protected from degradation and promoted shoot regeneration. All the prepared compounds were further tested for toxicity against Caenorhabditis elegans, and the assays revealed clear differences in toxicity between compounds with and without a hydroxyalkyl group. In a broader perspective, this work increases our understanding of CKX inhibition and provides a more extensive portfolio of compounds suitable for agricultural and biotechnological research. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa https://doi.org/10.1093/jxb/erac201
Počet záznamů: 1