Počet záznamů: 1
Performance of bat-derived macrophages at different temperatures
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SYSNO ASEP 0561727 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Performance of bat-derived macrophages at different temperatures Tvůrce(i) Němcová, M. (CZ)
Seidlová, Veronika (UBO-W)
Zukal, Jan (UBO-W) RID, ORCID, SAI
Dundarova, H. (BG)
Zukalová, K. (CZ)
Pikula, J. (CZ)Celkový počet autorů 6 Číslo článku 978756 Zdroj.dok. Frontiers in Veterinary Science. - : Frontiers Media
Roč. 9, SEP (2022)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Chiroptera (bats) ; in vitro model ; hibernation ; phagocytic activity ; temperature-dependent proliferation ; daily torpor ; macrophage biology Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Obor OECD Zoology Způsob publikování Open access Institucionální podpora UBO-W - RVO:68081766 UT WOS 000858548200001 EID SCOPUS 85138833530 DOI 10.3389/fvets.2022.978756 Anotace Heterothermy, as a temperature-dependent physiological continuum, may affect host-pathogen interactions through modulation of immune responses. Here, we evaluated proliferation and functional performance of a macrophage cell line established from the greater mouse-eared (Myotis myotis) bat at 8, 17.5, and 37 degrees C to simulate body temperatures during hibernation, daily torpor and euthermia. Macrophages were also frozen to20 degrees C and then examined for their ability to proliferate in the immediate post-thaw period. We show that bat macrophages can proliferate at lower temperatures, though their growth rate is significantly slower than at 37 degrees C. The cells differed in their shape, size and ability to attach to the plate surface at both lower temperatures, being spheroidal and free in suspension at 8 degrees C and epithelial-like, spindle-shaped and/or spheroidal at 17.5 degrees C. While phagocytosis at temperatures of 8 and 17.5 degrees C amounted to 85.8 and 83.1% of the activity observed at 37 degrees C, respectively, full phagocytic activity was restored within minutes of translocation into a higher temperature. Bat-derived macrophages were also able to withstand temperatures of 20 degrees C in a cryoprotectant-free cultivation medium and, in the immediate post-thaw period, became viable and were able to proliferate. Our in vitro data enhance understanding of macrophage biology. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2023 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fvets.2022.978756/full
Počet záznamů: 1