Počet záznamů: 1
Isolation and Characterization of Two Lytic Phages Efficient Against Phytopathogenic Bacteria From Pseudomonas and Xanthomonas Genera
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SYSNO ASEP 0561305 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Isolation and Characterization of Two Lytic Phages Efficient Against Phytopathogenic Bacteria From Pseudomonas and Xanthomonas Genera Tvůrce(i) Korniienko, Nataliia (UEB-Q) ORCID
Kharina, A. (UA)
Zrelovs, N. (LT)
Jindřichová, Barbora (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Budzanivska, I. (UA)
Burketová, Lenka (UEB-Q) RID, ORCID
Kalachova, Tetiana (UEB-Q) ORCIDCelkový počet autorů 8 Číslo článku 853593 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 13, APR 25 (2022)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Autographiviridae ; bacteriophage (phage) ; biocontrol ; phytopathogenic bacteria ; Pseudomonas syringae pv. tomato Obor OECD Biochemical research methods CEP EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000805882200001 EID SCOPUS 85129953702 DOI 10.3389/fmicb.2022.853593 Anotace Pseudomonas syringae is a bacterial pathogen that causes yield losses in various economically important plant species. At the same time, P. syringae pv. tomato (Pst) is one of the best-studied bacterial phytopathogens and a popular model organism. In this study, we report on the isolation of two phages from the market-bought pepper fruit showing symptoms of bacterial speck. These Pseudomonas phages were named Eir4 and Eisa9 and characterized using traditional microbiological methods and whole-genome sequencing followed by various bioinformatics approaches. Both of the isolated phages were capable only of the lytic life cycle and were efficient against several pathovars from Pseudomonas and Xanthomonas genera. With the combination of transmission electron microscopy (TEM) virion morphology inspection and comparative genomics analyses, both of the phages were classified as members of the Autographiviridae family with different degrees of novelty within the known phage diversity. Eir4, but not Eisa9, phage application significantly decreased the propagation of Pst in the leaf tissues of Arabidopsis thaliana plants. The biological properties of Eir4 phage allow us to propose it as a potential biocontrol agent for use in the prevention of Pst-associated bacterioses and also as a model organism for the future research of mechanisms of phage–host interactions in different plant systems. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa https://doi.org/10.3389/fmicb.2022.853593
Počet záznamů: 1