Počet záznamů: 1
Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations
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SYSNO ASEP 0561238 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations Tvůrce(i) Šolínová, Veronika (UOCHB-X) RID, ORCID
Tůma, P. (CZ)
Butnariu, Maria (UOCHB-X) ORCID
Kašička, Václav (UOCHB-X) RID, ORCID
Koval, Dušan (UOCHB-X) RID, ORCIDZdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 43, č. 20 (2022), s. 1953-1962Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova capillary coating ; capillary electrophoresis ; electroosmotic flow ; chiral separation ; helicene Obor OECD Analytical chemistry CEP GA20-03899S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000850177300001 EID SCOPUS 85138286705 DOI 10.1002/elps.202200130 Anotace We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2-acrylamido-2-methyl-1-propanesulfonate (AMPS), that is, poly(AM-co-AMPS) or PAMAMPS. Coatings were formed by the in-capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to similar to 40 x 10(-9) m(2) V-1 s(-1) interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS-treated capillaries, we observed (i) a negligible dependence on pH in the 2-10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter-EOF mode. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023 Elektronická adresa https://doi.org/10.1002/elps.202200130
Počet záznamů: 1