Počet záznamů: 1
Kinetic Study of 17 alpha-Estradiol Mechanism during Rat Sperm Capacitation
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SYSNO ASEP 0559402 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Kinetic Study of 17 alpha-Estradiol Mechanism during Rat Sperm Capacitation Tvůrce(i) Bosáková, Tereza (BTO-N)
Tockstein, A. (CZ)
Bosáková, Z. (CZ)
Komrsková, Kateřina (BTO-N) ORCIDCelkový počet autorů 4 Číslo článku 4092 Zdroj.dok. Molecules. - : MDPI
Roč. 27, č. 13 (2022)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova 17 alpha-estradiol ; estrogen receptors ; rat ; sperm ; capacitation ; hplc ms/ms ; kinetics Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GC20-20217J GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000824750800001 EID SCOPUS 85133232087 DOI 10.3390/molecules27134092 Anotace 17 alpha-Estradiol (alpha E2) is a natural diastereoisomer of 17 beta-estradiol (E2). It is well known that alpha E2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of alpha E2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added alpha E2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations B-t were subjected to kinetic analysis. The findings indicated that alpha E2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1420-3049/27/13/4092
Počet záznamů: 1