Počet záznamů: 1
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.
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SYSNO ASEP 0557084 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Tvůrce(i) Sedláček, I. (CZ)
Holochová, P. (CZ)
Busse, H. (AT)
Koublová, V. (CZ)
Králová, S. (CZ)
Švec, P. (CZ)
Sobotka, Roman (MBU-M) RID, ORCID
Staňková, E. (CZ)
Pilný, Jan (MBU-M) ORCID
Šedo, O. (CZ)
Smolíková, J. (CZ)
Sedlář, K. (CZ)Číslo článku 704 Zdroj.dok. Microorganisms. - : MDPI
Roč. 10, č. 4 (2022)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova emended description ; lipid-composition ; bacteria ; genome ; database ; soil ; gene ; classification ; degradation ; restriction ; Massilia ; violacein ; psychrophilic ; description ; whole-genome sequencing ; Antarctica Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Výzkumná infrastruktura CIISB II - 90127 - Masarykova univerzita
CzechPolar2 - 90078 - Masarykova univerzitaZpůsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000786017300001 EID SCOPUS 85126958615 DOI 10.3390/microorganisms10040704 Anotace A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398(T) were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398(T) represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398(T) (= CCM 8941(T) = LMG 32108(T)). Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/2076-2607/10/4/704
Počet záznamů: 1