Počet záznamů: 1
Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules
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SYSNO ASEP 0556422 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules Tvůrce(i) Fojtik, L. (CZ)
Fiala, J. (CZ)
Pompach, Petr (BTO-N)
Chmelík, J. (CZ)
Matoušek, V. (CZ)
Beier, P. (CZ)
Kukačka, Z. (CZ)
Novák, P. (CZ)Celkový počet autorů 8 Zdroj.dok. Journal of the American Chemical Society. - : American Chemical Society - ISSN 0002-7863
Roč. 143, č. 49 (2021), s. 20670-20679Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova fast photochemical oxidation ; hypervalent iodine reagents ; mass-spectrometry ; top-down ; myoglobin Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000750799000004 EID SCOPUS 85120891502 DOI 10.1021/jacs.1c07771 Anotace Covalent labeling of proteins in combination with mass spectrometry has been established as a complementary technique to classical structural methods, such as X-ray, NMR, or cryogenic electron microscopy (Cryo-EM), used for protein structure determination. Although the current covalent labeling techniques enable the protein solvent accessible areas with sufficient spatial resolution to be monitored, there is still high demand for alternative, less complicated, and inexpensive approaches. Here, we introduce a new covalent labeling method based on fast fluoroalkylation of proteins (FFAP). FFAP uses fluoroalkyl radicals formed by reductive decomposition of Togni reagents with ascorbic acid to label proteins on a time scale of seconds. The feasibility of FFAP to effectively label proteins was demonstrated by monitoring the differential amino acids modification of native horse heart apomyoglobin/holomyoglobin and the human haptoglobin-hemoglobin complex. The obtained data confirmed the Togni reagent-mediated FFAP is an advantageous alternative method for covalent labeling in applications such as protein footprinting and epitope mapping of proteins (and their complexes) in general. Data are accessible via the ProteomeXchange server with the data set identifier PXD027310. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2022 Elektronická adresa https://pubs.acs.org/doi/10.1021/jacs.1c07771
Počet záznamů: 1