Počet záznamů: 1
Extracellular glycolytic activities in root endophytic Serendipitaceae and their regulation by plant sugars
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SYSNO ASEP 0555990 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extracellular glycolytic activities in root endophytic Serendipitaceae and their regulation by plant sugars Tvůrce(i) De Rocchis, V. (DE)
Roitsch, Thomas (UEK-B) RID, ORCID, SAI
Franken, P. (DE)Celkový počet autorů 3 Číslo článku 320 Zdroj.dok. Microorganisms. - : MDPI
Roč. 10, č. 2 (2022)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova piriformospora-indica ; cell-wall ; monosaccharide transporters ; carbohydrate-metabolism ; arbuscular mycorrhiza ; protein secretion ; bacillus-subtilis ; filamentous fungi ; down-regulation ; l-arabinose ; endophytic fungi ; sugar metabolism ; glycolysis Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora UEK-B - RVO:86652079 UT WOS 000762506500001 EID SCOPUS 85123529218 DOI 10.3390/microorganisms10020320 Anotace Endophytic fungi that colonize the plant root live in an environment with relative high concentrations of different sugars. Analyses of genome sequences indicate that such endophytes can secrete carbohydrate-related enzymes to compete for these sugars with the surrounding plant cells. We hypothesized that typical plant sugars can be used as carbon source by root endophytes and that these sugars also serve as signals to induce the expression and secretion of glycolytic enzymes. The plant-growth-promoting endophytes Serendipita indica and Serendipita herbamans were selected to first determine which sugars promote their growth and biomass formation. Secondly, particular sugars were added to liquid cultures of the fungi to induce intracellular and extracellular enzymatic activities which were measured in mycelia and culture supernatants. The results showed that both fungi cannot feed on melibiose and lactose, but instead use glucose, fructose, sucrose, mannose, arabinose, galactose and xylose as carbohydrate sources. These sugars regulated the cytoplasmic activity of glycolytic enzymes and also their secretion. The levels of induction or repression depended on the type of sugars added to the cultures and differed between the two fungi. Since no conventional signal peptide could be detected in most of the genome sequences encoding the glycolytic enzymes, a non-conventional protein secretory pathway is assumed. The results of the study suggest that root endophytic fungi translocate glycolytic activities into the root, and this process is regulated by the availability of particular plant sugars. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/2076-2607/10/2/320
Počet záznamů: 1