Počet záznamů: 1
Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex
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SYSNO ASEP 0555830 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex Tvůrce(i) Ujčíková, Hana (FGU-C) RID, ORCID
Eckhardt, Adam (FGU-C) RID, ORCID
Hejnová, L. (CZ)
Novotný, J. (CZ)
Svoboda, Petr (FGU-C) RID, ORCIDČíslo článku 80 Zdroj.dok. Biomedicines. - : MDPI
Roč. 10, č. 1 (2022)Poč.str. 22 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova protracted morphine withdrawal ; rat hippocampus ; rat brain cortex ; gel-based proteomics ; energy metabolism ; oxidative stress ; nLC-MS/MS Obor OECD Physiology (including cytology) CEP GA19-03295S GA ČR - Grantová agentura ČR Výzkumná infrastruktura C4SYS - 90055 - Mikrobiologický ústav AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000747786400001 EID SCOPUS 85122254364 DOI 10.3390/biomedicines10010080 Anotace The knowledge about proteome changes proceeding during protracted opioid withdrawal is lacking. Therefore, the aim of this work was to analyze the spectrum of altered proteins in the rat hippocampus in comparison with the forebrain cortex after 6-month morphine withdrawal. We utilized 2D electrophoretic workflow (Pro-Q(R) Diamond staining and Colloidal Coomassie Blue staining) which was preceded by label-free quantification (MaxLFQ). The phosphoproteomic analysis revealed six significantly altered hippocampal (Calm1, Ywhaz, Tuba1b, Stip1, Pgk1, and Aldoa) and three cortical proteins (Tubb2a, Tuba1a, and Actb). The impact of 6-month morphine withdrawal on the changes in the proteomic profiles was higher in the hippocampus-14 proteins, only three proteins were detected in the forebrain cortex. Gene Ontology (GO) enrichment analysis of differentially expressed hippocampal proteins revealed the most enriched terms related to metabolic changes, cytoskeleton organization and response to oxidative stress. There is increasing evidence that energy metabolism plays an important role in opioid addiction. However, the way how morphine treatment and withdrawal alter energy metabolism is not fully understood. Our results indicate that the rat hippocampus is more susceptible to changes in proteome and phosphoproteome profiles induced by 6-month morphine withdrawal than is the forebrain cortex. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/2227-9059/10/1/80
Počet záznamů: 1