0555830 - FGÚ 2023 RIV CH eng J - Článek v odborném periodiku
Ujčíková, Hana - Eckhardt, Adam - Hejnová, L. - Novotný, J. - Svoboda, Petr
Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex.
Biomedicines. Roč. 10, č. 1 (2022), č. článku 80. E-ISSN 2227-9059
Grant CEP: GA ČR(CZ) GA19-03295S
Výzkumná infrastruktura: C4SYS - 90055
Institucionální podpora: RVO:67985823
Klíčová slova: protracted morphine withdrawal * rat hippocampus * rat brain cortex * gel-based proteomics * energy metabolism * oxidative stress * nLC-MS/MS
Obor OECD: Physiology (including cytology)
Impakt faktor: 4.7, rok: 2022
Způsob publikování: Open access
Web výsledku:
https://www.mdpi.com/2227-9059/10/1/80
DOI: https://doi.org/10.3390/biomedicines10010080

The knowledge about proteome changes proceeding during protracted opioid withdrawal is lacking. Therefore, the aim of this work was to analyze the spectrum of altered proteins in the rat hippocampus in comparison with the forebrain cortex after 6-month morphine withdrawal. We utilized 2D electrophoretic workflow (Pro-Q(R) Diamond staining and Colloidal Coomassie Blue staining) which was preceded by label-free quantification (MaxLFQ). The phosphoproteomic analysis revealed six significantly altered hippocampal (Calm1, Ywhaz, Tuba1b, Stip1, Pgk1, and Aldoa) and three cortical proteins (Tubb2a, Tuba1a, and Actb). The impact of 6-month morphine withdrawal on the changes in the proteomic profiles was higher in the hippocampus-14 proteins, only three proteins were detected in the forebrain cortex. Gene Ontology (GO) enrichment analysis of differentially expressed hippocampal proteins revealed the most enriched terms related to metabolic changes, cytoskeleton organization and response to oxidative stress. There is increasing evidence that energy metabolism plays an important role in opioid addiction. However, the way how morphine treatment and withdrawal alter energy metabolism is not fully understood. Our results indicate that the rat hippocampus is more susceptible to changes in proteome and phosphoproteome profiles induced by 6-month morphine withdrawal than is the forebrain cortex.

Trvalý link: http://hdl.handle.net/11104/0330289