Počet záznamů: 1
Transcription profiles in BEAS-2B cells exposed to organic extracts from particulate emissions produced by a port-fuel injection vehicle, fueled with conventional fossil gasoline and gasoline-ethanol blend
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SYSNO ASEP 0551564 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transcription profiles in BEAS-2B cells exposed to organic extracts from particulate emissions produced by a port-fuel injection vehicle, fueled with conventional fossil gasoline and gasoline-ethanol blend Tvůrce(i) Líbalová, Helena (UEM-P) RID
Závodná, Táňa (UEM-P)
Vrbová, Kristýna (UEM-P)
Sikorová, Jitka (UEM-P)
Vojtisek-Lom, M. (CZ)
Beránek, V. (CZ)
Pechout, Martin (UEM-P)
Kléma, J. (CZ)
Cigánek, M. (CZ)
Machala, M. (CZ)
Neca, J. (CZ)
Rössner ml., Pavel (UEM-P) RID, ORCID
Topinka, Jan (UEM-P) RID, ORCIDČíslo článku 503414 Zdroj.dok. Mutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718
Roč. 872, dec (2021)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova particulate matter emissions ; gasoline ; alternative fuels ; toxicity ; gene expression profiling Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Toxicology CEP GJ18-06548Y GA ČR - Grantová agentura ČR LM2018124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018133 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000708693800004 EID SCOPUS 85116939286 DOI 10.1016/j.mrgentox.2021.503414 Anotace Emissions from road traffic are among the major contributors to air pollution worldwide and represent a serious environmental health risk. Although traffic-related pollution has been most commonly associated with diesel engines, increasing evidence suggests that gasoline engines also produce a considerable amount of potentially hazardous particulate matter (PM). The primary objective of this study was to compare the intrinsic toxic properties of the organic components of PM, generated by a conventional gasoline engine fueled with neat gasoline (E0), or gasoline-ethanol blend (15 % ethanol, v/v, E15). Our results showed that while E15 has produced, compared to gasoline and per kg of fuel, comparable particle mass (mu g PM/kg fuel) and slightly more particles by number, the organic extract from the particulate matter produced by E15 contained a larger amount of harmful polycyclic aromatic hydrocarbons (PAHs), as determined by the chemical analysis. To examine the toxicity, we monitored genome-wide gene expression changes in human lung BEAS-2B cells, exposed for 4 h and 24 h to a subtoxic dose of each PM extract. After 4 h exposure, numerous dysregulated genes and processes such as oxidative stress, lipid and steroid metabolism, PPAR alpha signaling and immune response, were found to be common for both extract treatments. On the other hand, 24 h exposure resulted in more distinctive gene expression patterns. Although we identified several common modulated processes indicating the metabolism of PAHs and activation of aryl hydrocarbon receptor (AhR), E15 specifically dysregulated a variety of other genes and pathways related to cancer promotion and progression. Overall, our findings suggest that the ethanol addition to gasoline changed the intrinsic properties of PM emissions and increased the PAH content in PM organic extract, thus contributing to a more extensive toxic response particularly after 24 h exposure in BEAS-2B cells. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1383571821001054?via%3Dihub
Počet záznamů: 1