Počet záznamů: 1
In-bone protein digestion followed by LC-MS/MS peptide analysis as a new way towards the routine proteomic characterization of human maxillary and mandibular bone tissue in oral surgery
- 1.
SYSNO ASEP 0545929 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In-bone protein digestion followed by LC-MS/MS peptide analysis as a new way towards the routine proteomic characterization of human maxillary and mandibular bone tissue in oral surgery Tvůrce(i) Hynek, R. (CZ)
Michalus, I. (CZ)
Cejnar, P. (CZ)
Šantrůček, J. (CZ)
Seidlová, S. (CZ)
Kučková, Š. (CZ)
Sázelová, Petra (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 42, č. 23 (2021), s. 2552-2562Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova in-bone protein digestion ; LC-MS/MS ; mandibular bone ; maxillary bone ; oral surgery ; peptide mapping Obor OECD Analytical chemistry CEP GA18-02597S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000695024600001 EID SCOPUS 85114698208 DOI 10.1002/elps.202100211 Anotace Proteomic characterization of alveolar bones in oral surgery represents an analytical challenge due to their insoluble character. The implementation of a straightforward technique could lead to the routine use of proteomics in this field. This work thus developed a simple technique for the characterization of bone tissue for human maxillary and mandibular bones. It is based on the direct in-bone tryptic digestion of proteins in both healthy and pathological human maxillary and mandibular bone samples. The released peptides were then identified by the LC-MS/MS. Using this approach, a total of 1120 proteins were identified in the maxillary bone and 1151 proteins in the mandibular bone. The subsequent partial least squares–discrimination analysis (PLS-DA) of protein data made it possible to reach 100% discrimination between the samples of healthy alveolar bones and those of the bone tissue surrounding the inflammatory focus. These results indicate that the in-bone protein digestion followed by the LC-MS/MS and subsequent statistical analysis can provide a deeper insight into the field of oral surgery at the molecular level. Furthermore, it could also have a diagnostic potential in the differentiation between the proteomic patterns of healthy and pathological alveolar bone tissue. Data are available via ProteomeXchange with the identifier PXD026775. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2022 Elektronická adresa https://doi.org/10.1002/elps.202100211
Počet záznamů: 1