Počet záznamů: 1
PacBio sequencing of Glomeromycota rDNA: a novel amplicon covering all widely used ribosomal barcoding regions and its applicability in taxonomy and ecology of arbuscular mycorrhizal fungi
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SYSNO ASEP 0545683 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název PacBio sequencing of Glomeromycota rDNA: a novel amplicon covering all widely used ribosomal barcoding regions and its applicability in taxonomy and ecology of arbuscular mycorrhizal fungi Tvůrce(i) Kolaříková, Zuzana (BU-J) ORCID, RID
Slavíková, Renata (BU-J)
Krüger, Claudia (BU-J) ORCID
Krüger, Manuela (BU-J) ORCID
Kohout, Petr (BU-J) ORCIDZdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 231, č. 1 (2021), s. 490-499Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Archaeosporales ; long-read metabarcoding ; PacBio Vědní obor RIV EF - Botanika Obor OECD Mycology CEP GA17-03921S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BU-J - RVO:67985939 UT WOS 000645953600001 EID SCOPUS 85105234417 DOI 10.1111/nph.17372 Anotace Using a nested PCR approach specific to AMF, we amplified a part (c. 2.5 kb) of the rDNA spanning the majority of the small subunit rRNA (SSU) gene, the complete internal transcribed spacer (ITS) region and a part of the large subunit (LSU) rRNA gene. The PCR products were sequenced on the PacBio platform utilizing Single Molecule Real Time (SMRT) sequencing. Employing this method for selected environmental DNA samples, we were able to describe complex AMF communities consisting of various glomeromycotan lineages. We demonstrate the applicability of this new 2.5 kb approach to provide robust phylogenetic assignment of AMF lineages without known sequences from pure cultures and to consolidate information about AMF taxon distributions coming from three widely used barcoding regions into one integrative dataset. Pracoviště Botanický ústav Kontakt Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Rok sběru 2022 Elektronická adresa https://doi.org/10.1111/nph.17372
Počet záznamů: 1