Počet záznamů: 1
Fluorescent Probe for Selective Imaging of α-Synuclein Fibrils in Living Cells
- 1.0542333 - ÚOCHB 2022 RIV US eng J - Článek v odborném periodiku
Gaur, Pankaj - Galkin, Maksym - Kurochka, Andrii - Ghosh, S. - Yushchenko, Dmytro A. - Shvadchak, Volodymyr V.
Fluorescent Probe for Selective Imaging of α-Synuclein Fibrils in Living Cells.
ACS Chemical Neuroscience. Roč. 12, č. 8 (2021), s. 1293-1298. ISSN 1948-7193. E-ISSN 1948-7193
Grant CEP: GA ČR(CZ) GA19-21318S; GA ČR(CZ) GJ18-06255Y
Institucionální podpora: RVO:61388963
Klíčová slova: fluorescence * α-synuclein * fibril * microscopy * solvatochromic
Obor OECD: Biophysics
Impakt faktor: 5.780, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1021/acschemneuro.1c00090
Plaques of amyloid fibrils composed of neuronal protein α-synuclein are one of the hallmarks of Parkinson’s disease, and their selective imaging is crucial to study the mechanism of its pathogenesis. However, the existing fluorescent probes for amyloids are efficient only in solution and tissue systems, and they are not selective enough for the visualization of amyloid fibrils in living cells. In this study, we present two molecular rotor-based probes RB1 and RB2. These thiazolium probes show affinity to α-synuclein fibrils and turn-on fluorescence response upon interactions. Because of its extended π-conjugation and high rotational degree of freedom, RB1 exhibits a 76 nm red-shift of absorption maxima and 112-fold fluorescence enhancement upon binding to amyloid fibrils. Owing to its strong binding affinity to α-synuclein fibrils, RB1 can selectively stain them in the cytoplasm of living HeLa and SH-SY5Y cells with high optical contrast. RB1 is a cell-permeable and noncytotoxic probe. Taken together, we have demonstrated that RB1 is an amyloid probe with an outstanding absorption red-shift that can be used for intracellular imaging of α-synuclein fibrils.
Trvalý link: http://hdl.handle.net/11104/0319764
Počet záznamů: 1