Počet záznamů: 1
Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes
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SYSNO ASEP 0538171 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes Tvůrce(i) Králová, Jarmila (UMG-J) RID
Jurášek, M. (CZ)
Miksatkova, L. (CZ)
Marešová, A. (CZ)
Faehnrich, J. (CZ)
Cihlářová, P. (CZ)
Drašar, P. (CZ)
Bartůněk, Petr (UMG-J) RID
Král, V. (CZ)Celkový počet autorů 9 Číslo článku 22053 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova cholesterol analogs ; bodipy Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP GA17-02836S GA ČR - Grantová agentura ČR LM2018130 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000603657800019 DOI 10.1038/s41598-020-78085-9 Anotace Fluorescent sterol probes, comprising a fluorophore connected to a sterol backbone by means of a linker, are promising tools for enabling high-resolution imaging of intracellular cholesterol. In this study, we evaluated how the size of the linker, site of its attachment and nature of the fluorophore, affect the localization and trafficking properties of fluorescent sterol probes. Varying lengths of linker using the same fluorophore affected cell penetration and retention in specific cell compartments. A C-4 linker was confirmed as optimal. Derivatives of heterocyclic sterol precursors attached with identical C-4 linker to different fluorophores at diverse positions also showed significant differences in their binding properties to various intracellular compartments and kinetics of trafficking. Two novel red-emitting probes with good cell permeability, fast intracellular labelling and slightly different distribution displayed very promising characteristics for sterol probes. These probes also strongly labelled endo/lysosomal compartment in cells with pharmacologically disrupted cholesterol transport, or with a genetic mutation of cholesterol transporting protein NPC1, that overlapped with filipin staining of cholesterol. Overall, the present study demonstrates that the physicochemical properties of the fluorophore/linker pairing determine the kinetics of uptake and distribution and subsequently influence the applicability of final probes. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2021 Elektronická adresa https://www.nature.com/articles/s41598-020-78085-9
Počet záznamů: 1