Počet záznamů: 1
Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae.
- 1.
SYSNO ASEP 0532455 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae. Tvůrce(i) Robledillo, Laura Avila (BC-A) RID, ORCID
Neumann, Pavel (BC-A) RID, ORCID
Koblížková, Andrea (BC-A)
Novák, Petr (BC-A) RID, ORCID
Vrbová, Iva (BC-A) RID, ORCID
Macas, Jiří (BC-A) RID, ORCID, SAICelkový počet autorů 6 Zdroj.dok. Molecular Biology and Evolution. - : Oxford University Press - ISSN 0737-4038
Roč. 37, č. 8 (2020), s. 2341-2356Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova centromere evolution ; satellite DNA ; CENH3 ; ChIP-seq ; plant chromosomes Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GA17-09750S GA ČR - Grantová agentura ČR LM2015047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000574381000016 EID SCOPUS 85089203169 DOI https://doi.org/10.1093/molbev/msaa090 Anotace Satellite repeats are major sequence constituents of centromeres in many plant and animal species. Within a species, a single family of satellite sequences typically occupies centromeres of all chromosomes and is absent from other parts of the genome. Due to their common origin, sequence similarities exist among the centromere-specific satellites in related species. Here, we report a remarkably different pattern of centromere evolution in the plant tribe Fabeae, which includes genera Pisum, Lathyrus, Vicia, and Lens. By immunoprecipitation of centromeric chromatin with CENH3 antibodies, we identified and characterized a large and diverse set of 64 families of centromeric satellites in 14 species. These families differed in their nucleotide sequence, monomer length (33-2,979bp), and abundance in individual species. Most families were species-specific, and most species possessed multiple (2-12) satellites in their centromeres. Some of the repeats that were shared by several species exhibited promiscuous patterns of centromere association, being located within CENH3 chromatin in some species, but apart from the centromeres in others. Moreover, FISH experiments revealed that the same family could assume centromeric and noncentromeric positions even within a single species. Taken together, these findings suggest that Fabeae centromeres are not shaped by the coevolution of a single centromeric satellite with its interacting CENH3 proteins, as proposed by the centromere drive model. This conclusion is also supported by the absence of pervasive adaptive evolution of CENH3 sequences retrieved from Fabeae species. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2021 Elektronická adresa https://academic.oup.com/mbe/article/37/8/2341/5817320
Počet záznamů: 1