Počet záznamů: 1
Tubulin response to intense nanosecond-scale electric field in molecular dynamics simulation
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SYSNO ASEP 0518300 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tubulin response to intense nanosecond-scale electric field in molecular dynamics simulation Tvůrce(i) Marracino, P. (IT)
Havelka, Daniel (URE-Y) RID
Průša, Jiří (URE-Y)
Liberti, M. (IT)
Tuszyński, J.A. (CA)
Ayoub, A.T. (EG)
Apollonio, F. (IT)
Cifra, Michal (URE-Y) RID, ORCID, SAICelkový počet autorů 8 Číslo článku 10477 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 9, č. 1 (2019)Poč.str. 14 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Electric fields ; Molecular dynamics ; Microwave heating Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika Obor OECD Electrical and electronic engineering CEP GA17-11898S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora URE-Y - RVO:67985882 UT WOS 000476468700005 EID SCOPUS 85069477341 DOI 10.1038/s41598-019-46636-4 Anotace Intense pulsed electric fields are known to act at the cell membrane level and are already being exploited in biomedical and biotechnological applications. However, it is not clear if electric pulses within biomedically-attainable parameters could directly influence intra-cellular components such as cytoskeletal proteins. If so, a molecular mechanism of action could be uncovered for therapeutic applications of such electric fields. To help clarify this question, we first identified that a tubulin heterodimer is a natural biological target for intense electric fields due to its exceptional electric properties and crucial roles played in cell division. Using molecular dynamics simulations, we then demonstrated that an intense - yet experimentally attainable - electric field of nanosecond duration can affect the b beta-tubulin's C-terminus conformations and also influence local electrostatic properties at the GTPase as well as the binding sites of major tubulin drugs site. Our results suggest that intense nanosecond electric pulses could be used for physical modulation of microtubule dynamics. Since a nanosecond pulsed electric field can penetrate the tissues and cellular membranes due to its broadband spectrum, our results are also potentially significant for the development of new therapeutic protocols Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2020 Elektronická adresa https://www.nature.com/articles/s41598-019-46636-4.pdf?origin=ppub
Počet záznamů: 1