Počet záznamů: 1
Modulation of NMDA-Mediated Clock Resetting in the Suprachiasmatic Nuclei of mPer2(Luc) Mouse by Endocannabinoids
- 1.
SYSNO ASEP 0503881 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Modulation of NMDA-Mediated Clock Resetting in the Suprachiasmatic Nuclei of mPer2(Luc) Mouse by Endocannabinoids Tvůrce(i) Sládek, Martin (FGU-C) RID, ORCID, SAI
Sumová, Alena (FGU-C) RID, ORCIDČíslo článku 361 Zdroj.dok. Frontiers in Physiology. - : Frontiers Research Foundation - ISSN 1664-042X
Roč. 10, Mar 29 (2019)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova circadian ; suprachiasmatic nucleus ; entrainment ; NMDA ; glutamate receptor ; endocannabinoids ; PER2::LUC ; phase response curve Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP GA17-14704S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000462817500001 EID SCOPUS 85066441717 DOI 10.3389/fphys.2019.00361 Anotace Light entrains the master circadian clock in the suprachiasmatic nucleus (SCN) predominantly through glutamatergic signaling via NMDA receptors. The magnitude and the direction of resulting phase shifts depend on timing of the photic stimulus. Previous reports based on behavioral and electrophysiological data suggested that endocannabinoids (EC) might reduce the ability of the SCN clock to respond to light. However, there is little direct evidence for the involvement of EC in entrainment of the rhythmic clock gene expression in the SCN. We have used luminescence recording of cultured SCN slices from mPer2(Luc) mice to construct a complete phase response curve (PRC) for NMDA receptor activation. The results demonstrated that NMDA administration phase-shifts the PER2 rhythm in a time-specific manner. A stable “singularity,” in the course of which the clock seemingly stops while the overall phase is caught between delays and advances, can occur in response to NMDA at a narrow interval during the PER2 level decrease. NMDA-induced phase delays were affected neither by the agonist (WIN 55,212-2 mesylate) nor by the antagonist (rimonabant hydrochloride) of EC receptors. However, the agonist significantly reduced the NMDA-induced phase advance of the clock, while the antagonist enhanced the phase advance, causing a shift in the sensitivity window of the SCN to NMDA. The modulation of EC signaling in the SCN had no effect by itself on the phase of the PER2 rhythm. The results provide evidence for a modulatory role of EC in photic entrainment of the circadian clock in the SCN. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.3389/fphys.2019.00361
Počet záznamů: 1