Počet záznamů: 1
Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing
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SYSNO ASEP 0495958 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing Tvůrce(i) Douglas, T.E.L. (BE)
Dziadek, M. (PL)
Gorodzha, S. (RU)
Lišková, Jana (FGU-C) RID, ORCID
Brackman, G. (BE)
Vanhoorne, V. (BE)
Vervaet, C. (BE)
Balcaen, L. (BE)
Garcia, M.d.R.F. (BE)
Boccaccini, A. R. (DE)
Weinhardt, V. (DE)
Baumbach, T. (DE)
Vanhaecke, F. (BE)
Coenye, T. (BE)
Bačáková, Lucie (FGU-C) RID, ORCID
Surmeneva, M. A. (RU)
Surmenev, R. A. (RU)
Cholewa-Kowalska, K. (PL)
Skirtach, A. G. (BE)Zdroj.dok. Journal of Tissue Engineering and Regenerative Medicine. - : Wiley - ISSN 1932-6254
Roč. 12, č. 6 (2018), s. 1313-1326Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova bone regeneration ; injectable biomaterial ; composite ; gellan gum ; bioglass ; bioactive glass Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP NV15-32497A GA MZd - Ministerstvo zdravotnictví Institucionální podpora FGU-C - RVO:67985823 UT WOS 000435945500001 EID SCOPUS 85048785792 DOI 10.1002/term.2654 Anotace Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration. In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles. This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass. Gelation occurred within 20min. Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff. X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8m, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels. The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5). A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5. Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5. All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid. The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5. The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
Počet záznamů: 1