Počet záznamů: 1
Long terminal repeats power evolution of genes and gene expression programs in mammalian oocytes and zygotes
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SYSNO ASEP 0486275 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Long terminal repeats power evolution of genes and gene expression programs in mammalian oocytes and zygotes Tvůrce(i) Franke, V. (HR)
Ganesh, Sravya (UMG-J)
Karlic, R. (HR)
Malík, Radek (UMG-J) RID
Pasulka, Josef (UMG-J)
Horvat, F. (HR)
Kuzman, M. (HR)
Fulka, Helena (UMG-J)
Černohorská, Markéta (UMG-J)
Urbanová, Jana (UMG-J)
Svobodová, Eliška (UMG-J)
Ma, J. (US)
Suzuki, Y. (JP)
Aoki, F. (JP)
Schultz, R. M. (US)
Vlahovicek, K. (HR)
Svoboda, Petr (UMG-J) RIDCelkový počet autorů 17 Zdroj.dok. Genome Research. - : Cold Spring Harbor Laboratory Press - ISSN 1088-9051
Roč. 27, č. 8 (2017), s. 1384-1394Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova murine endogenous retrovirus ; antarctic notothenioid fish ; embryonic stem-cells ; transposable elements ; mouse oocytes ; muerv-l ; preimplantation embryos ; methylation landscape ; regulatory networks ; dna methylation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Reproductive biology (medical aspects to be 3) CEP LO1419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 UT WOS 000406354300009 DOI 10.1101/gr.216150.116 Anotace Retrotransposons are 'copy-and-paste' insertional mutagens that substantially contribute to mammalian genome content. Retrotransposons often carry long terminal repeats (LTRs) for retrovirus-like reverse transcription and integration into the genome. We report an extraordinary impact of a group of LTRs from the mammalian endogenous retrovirus-related ERVL retrotransposon class on gene expression in the germline and beyond. In mouse, we identified more than 800 LTRs from ORR1, MT, MT2, and MLT families, which resemble mobile gene-remodeling platforms that supply promoters and first exons. The LTR-mediated gene remodeling also extends to hamster, human, and bovine oocytes. The LTRs function in a stage specific manner during the oocyte-to-embryo transition by activating transcription, altering protein-coding sequences, producing noncoding RNAs, and even supporting evolution of new protein-coding genes. These functions result, for example, in recycling processed pseudogenes into mRNAs or lncRNAs with regulatory roles. The functional potential of the studied LTRs is even higher, because we show that dormant LTR promoter activity can rescue loss of an essential upstream promoter. We also report a novel protein-coding gene evolution-D6Ertd527e-in which an MT LTR provided a promoter and the 5' exon with a functional start codon while the bulk of the protein-coding sequence evolved through a CAG repeat expansion. Altogether, ERVL LTRs provide molecular mechanisms for stochastically scanning, rewiring, and recycling genetic information on an extraordinary scale. ERVL LTRs thus offer means for a comprehensive survey of the genome's expression potential, tightly intertwining with gene expression and evolution in the germline. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1