Počet záznamů: 1
Effects of Infrared Optical Trapping on Saccharomyces cerevisiae in a Microfluidic System
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SYSNO ASEP 0481578 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effects of Infrared Optical Trapping on Saccharomyces cerevisiae in a Microfluidic System Tvůrce(i) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Jonáš, A. (TR)
Ježek, Jan (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDCelkový počet autorů 4 Číslo článku 2640 Zdroj.dok. Sensors. - : MDPI
Roč. 17, NOV (2017), s. 1-12Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova optical trapping ; microfluidics ; phototoxicity ; laser ; Saccharomyces cerevisiae Vědní obor RIV BH - Optika, masery a lasery Obor OECD Optics (including laser optics and quantum optics) CEP LO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 UT WOS 000416790500202 EID SCOPUS 85034860345 DOI 10.3390/s17112640 Anotace Baker’s yeast (Saccharomyces cerevisiae) represents a very popular single-celled eukaryotic model organism which has been studied extensively by various methods and whose genome has been completely sequenced. It was also among the first living organisms that were manipulated by optical tweezers and it is currently a frequent subject of optical micromanipulation experiments. We built a microfluidic system for optical trapping experiments with individual cells and used it for the assessment of cell tolerance to phototoxic stress. Using optical tweezers with the wavelength of 1064 nm, we trapped individual Saccharomyces cerevisiae cells for 15 min and, subsequently, observed
their stress response in specially designed microfluidic chambers over time periods of several hours by time-lapse video microscopy. We determined the time between successive bud formations after the exposure to the trapping light, took account of damaged cells, and calculated the population doubling period and cell areas for increasing trapping power at a constant trapping time. Our approach represents an attractive, versatile microfluidic platform for quantitative optical trapping experiments
with living cells. We demonstrate its application potential by assessing the limits for safe, non-invasive optical trapping of Saccharomyces cerevisiae with infrared laser light.Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2018 Elektronická adresa http://www.mdpi.com/1424-8220/17/11/2640
Počet záznamů: 1