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Human histone deacetylase 6 shows strong preference for tubulin dimers over assembled microtubules
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SYSNO ASEP 0480758 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Human histone deacetylase 6 shows strong preference for tubulin dimers over assembled microtubules Tvůrce(i) Škultétyová, Ĺubica (BTO-N) RID
Ustinova, Kseniya (BTO-N)
Kutil, Zsofia (BTO-N) RID, ORCID
Nováková, Zora (BTO-N) ORCID, RID
Pavlíček, Jiří (BTO-N) RID
Mikesova, Jana (BTO-N)
Trapl, Dalibor (BTO-N)
Baranová, Petra (BTO-N)
Havlínová, Barbora (BTO-N)
Hubálek, Martin (UOCHB-X) RID, ORCID
Lánský, Zdeněk (BTO-N)
Bařinka, Cyril (BTO-N) RID, ORCIDCelkový počet autorů 12 Číslo článku 11547 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, 2017 Sep 14 (2017)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ALPHA-TUBULIN ; IN-VIVO ; MOLECULAR-BASIS ; POSTTRANSLATIONAL MODIFICATION Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Vědní obor RIV – spolupráce Ústav organické chemie a biochemie - Biochemie CEP GA15-19640S GA ČR - Grantová agentura ČR GA15-17488S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 ; UOCHB-X - RVO:61388963 UT WOS 000410739000045 DOI 10.1038/s41598-017-11739-3 Anotace Human histone deacetylase 6 (HDAC6) is the major deacetylase responsible for removing the acetyl group from Lys40 of alpha-tubulin (alpha K40), which is located lumenally in polymerized microtubules. Here, we provide a detailed kinetic analysis of tubulin deacetylation and HDAC6/microtubule interactions using individual purified components. Our data unequivocally show that free tubulin dimers represent the preferred HDAC6 substrate, with a K-M value of 0.23 mu M and a deacetylation rate over 1,500-fold higher than that of assembled microtubules. We attribute the lower deacetylation rate of microtubules to both longitudinal and lateral lattice interactions within tubulin polymers. Using TIRF microscopy, we directly visualized stochastic binding of HDAC6 to assembled microtubules without any detectable preferential binding to microtubule tips. Likewise, indirect immunofluorescence microscopy revealed that microtubule deacetylation by HDAC6 is carried out stochastically along the whole microtubule length, rather than from the open extremities. Our data thus complement prior studies on tubulin acetylation and further strengthen the rationale for the correlation between tubulin acetylation and microtubule age. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2018
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