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Diabetes mellitus negatively affects male reproductive parameters in vivo
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SYSNO ASEP 0462997 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název Diabetes mellitus negatively affects male reproductive parameters in vivo Tvůrce(i) Valášková, Eliška (BTO-N)
Žatecká, Eva (BTO-N)
Pavlínková, Gabriela (BTO-N) RID, ORCID
Bohuslavová, Romana (BTO-N) RID
Dorosh, Andriy (BTO-N) RID
Elzeinová, Fatima (BTO-N) RID
Kubátová, Alena (BTO-N) RID
Margaryan, Hasmik (BTO-N) RID
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 9 Zdroj.dok. Book of abstracts of XXIst Symposium of biology and immunology of reproduction. - Praha : Biotechnologický ústav AVČR, 2015 / Pěknicová Jana ; Elzeinová Fatima ; Kubátová Alena
S. 32-32Poč.str. 1 s. Akce XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION Datum konání 14.05.2015-16.05.2015 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova diabetes melitus ; Sterptozotocin ; apoptosis ; protamination Vědní obor RIV CE - Biochemie CEP GA P503/12/1834 GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 Anotace According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% of cases are caused by male factor. This could be caused by genetic background, environmental factors and various diseases, including diabetes mellitus (DM). However, the impact of DM on male fertility is not fully understood. . The aim of this study was to investigate the effects of DM on reproductive parameters and sperm quality, using mouse model. DM (type 1) was induced by Streptozotocin in FVB inbred mouse strain. Mice with blood sugar levels higher than 13.9 mmol/L were considered diabetic. After 4 weeks of diabetes exposure, diabetic males were bred with wild type females and transgenerational effect of DM was assessed. Selected morphological, cellular, and molecular parameters of diabetic males and their male offspring were compared to appropriate controls. There was an increased in sperm fragmentation and abnormalities of sperm morphology in diabetic mice in both generations. An increased staining with apoptotic marker annexin V was also detected in the diabetic groups. Furthermore, a presence of protamines as major sperm nuclear proteins was analysed. Protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was altered in sperms of experimental diabetic animals in both generations. Our findings indicate that DM type 1 negatively affects sperm quality and P1/P2 ratio and this negative effect is transmitted to the progeny Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
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