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Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
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SYSNO ASEP 0456423 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3 Tvůrce(i) Osička, Radim (MBU-M) RID, ORCID
Osičková, Adriana (MBU-M) RID, ORCID
Hasan, Shakir (MBU-M)
Bumba, Ladislav (MBU-M) RID, ORCID
Černý, Jiří (BTO-N) RID, ORCID
Šebo, Peter (MBU-M) RID, ORCIDZdroj.dok. eLife. - : eLife - ISSN 2050-084X
Roč. 4, DEC 9 (2015)Poč.str. 28 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova E. coli ; adenylate cyclase toxin ; biochemistry Vědní obor RIV CE - Biochemie CEP GAP302/11/0580 GA ČR - Grantová agentura ČR GA15-11851S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 UT WOS 000373901900001 DOI 10.7554/eLife.10766 Anotace Integrins are heterodimeric cell surface adhesion and signaling receptors that are essential for metazoan existence. Some integrins contain an I-domain that is a major ligand binding site. The ligands preferentially engage the active forms of the integrins and trigger signaling cascades that alter numerous cell functions. Here we found that the adenylate cyclase toxin (CyaA), a key virulence factor of the whooping cough agent Bordetella pertussis, preferentially binds an inactive form of the integrin complement receptor 3 (CR3), using a site outside of its I-domain. CyaA binding did not trigger downstream signaling of CR3 in human monocytes and CyaAcatalyzed elevation of cAMP effectively blocked CR3 signaling initiated by a natural ligand. This unprecedented type of integrin-ligand interaction distinguishes CyaA from all other known ligands of the I-domain-containing integrins and provides a mechanistic insight into the previously observed central role of CyaA in the pathogenesis of B. pertussis. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2016
Počet záznamů: 1