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Voltammetric Study of dsDNA Modified by Multi-redox Label Based on N-methyl-4-hydrazino-7-nitrobenzofurazan
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SYSNO ASEP 0435356 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Voltammetric Study of dsDNA Modified by Multi-redox Label Based on N-methyl-4-hydrazino-7-nitrobenzofurazan Tvůrce(i) Daňhel, Aleš (BFU-R) RID, ORCID
Raindlová, Veronika (UOCHB-X) RID
Havran, Luděk (BFU-R) RID, ORCID
Barek, J. (CZ)
Hocek, Michal (UOCHB-X) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 129, May 20 (2014), s. 348-357Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova DNA ; redox labeling ; N-methyl-4-hydrazino-7-nitrobenzofurazan ; silver solid amalgam electrode ; voltammetry Vědní obor RIV CC - Organická chemie Vědní obor RIV – spolupráce Biofyzikální ústav - Biofyzika CEP GBP206/12/G151 GA ČR - Grantová agentura ČR EE2.3.30.0019 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UOCHB-X - RVO:61388963 ; BFU-R - RVO:68081707 UT WOS 000336466100045 EID SCOPUS 84896912789 DOI 10.1016/j.electacta.2014.02.137 Anotace Voltammetric behaviour of novel synthetic multi-redox DNA label N-methyl-4-hydrazino-7-nitrobenzofurazan (NBF), a deoxycytidine monophosphate conjugate with NBF via formylthiophene linker (dC(NBF)MP), and NBF labeled model short double stranded DNA (dsDNA-NBF) were systematically studied at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) for the first time in this work. Successful enzymatic incorporation of the NBF labeled deoxycytidine triphosphate into the DNA was confirmed and the dsDNA-NBF offered new more positive cathodic signals at -520 mV and 1160 mV, compared to the nucleic acid itself. Used modification resulted in increase of selectivity and sensitivity of the DNA voltammetric determination. Next to the pioneering voltammetric behaviour of all studied compounds at m-AgSAE, electroanalytical methods based on cyclic voltammetry, adsorptive stripping cyclic voltammetry and open circuit transfer stripping cyclic voltammetry were developed for sensitive detection of these (bio)molecules. Furthermore, utilization of variable negative vertex potential and scan rate was also observed and their appropriate selection considerably influenced registered peaks on cyclic voltammograms. These redox processes increased signal response diversity and offered utilization of signal switch on/off technique, as well. Tentative electrochemical reduction mechanisms of the NBF, dC(NBF)MP and dsDNA-NBF were proposed and discussed, together with possibilities and limitations of the NBF + m-AgSAE system. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2015
Počet záznamů: 1