Počet záznamů: 1
Loss of membrane fluidity and endocytosis inhibition are involved in rapid aluminum-induced root growth cessation in Arabidopsis thaliana
- 1.
SYSNO ASEP 0380983 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Loss of membrane fluidity and endocytosis inhibition are involved in rapid aluminum-induced root growth cessation in Arabidopsis thaliana Tvůrce(i) Krtková, J. (CZ)
Havelková, L. (CZ)
Křepelová, A. (CZ)
Fišer, R. (CZ)
Vosolsobě, S. (CZ)
Novotná, Z. (CZ)
Martinec, Jan (UEB-Q) RID, ORCID
Schwarzerová, K. (CZ)Zdroj.dok. Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 60, Nov 2012 (2012), s. 88-97Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova Aluminum toxicity ; Arabidopsis thaliana ; Cortical microtubules Vědní obor RIV ED - Fyziologie CEP GA522/05/0340 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000310387400012 DOI 10.1016/j.plaphy.2012.07.030 Anotace Aluminum (Al) toxicity is the main limiting factor in crop production on acid soils. The main symptom of Al toxicity is a rapid inhibition of root growth, but the mechanism of root growth cessation remains unclear. Here we examined the earliest changes in the plasma membrane and processes related to the membrane in the Arabidopsis thaliana root tip cells of roots grown in a hydropony. Al suppressed root growth within 2 min, inhibited endocytosis within 10 min of exposure and stabilized cortical microtubules within the first 30 min. Spectrofluorometric measurements of the plasma membrane isolated from Arabidopsis plants and labeled with the fluorescent probe laurdan showed that Al induced a reduction in membrane fluidity. Application of the membrane fluidizer, benzyl alcohol, restored partially membrane fluidity and also partially restored root growth during first 30 min of Al treatment. We concluded that Al-induced loss of membrane fluidity and endocytosis inhibition occurred very early during Al toxicity in plant roots and could be the earliest targets of Al treatment. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1