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CDC25A is able to induce resumption of meiosis but compromises metaphase I-metaphase II transition in mouse oocytes
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SYSNO ASEP 0089293 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Stať ve sborníku Název CDC25A is able to induce resumption of meiosis but compromises metaphase I-metaphase II transition in mouse oocytes Překlad názvu CDC25A je schopna indukovat znovuzahájení meiosy, ale inhibuje metafase I – metafase II přechod Tvůrce(i) Šolc, Petr (UZFG-Y) RID, ORCID
Šašková, Adéla (UZFG-Y)
Baran, V. (SK)
Kubelka, Michal (UZFG-Y) RID, ORCID
Motlík, Jan (UZFG-Y) RID, ORCIDZdroj.dok. Programme and book of abstracts of Conference on Reproductive and Developmental Biology. - Mělník : ÚŽFG, 2007
S. 9-9Poč.str. 1 s. Akce Conference on Reproductive and Developmental Biology Datum konání 21.06.2007-22.06.2007 Místo konání Praha Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova meiosis Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50450515 - UZFG-Y (2005-2011) Anotace We have shown that CDC25A protein is expressed in GV-stage oocytes but decreases, in CDK1-dependent manner, during meiotic maturation. As compared with GV-stage only a very low level of CDC25A protein is present at metaphase I (MI) and metaphase II (MII) stages. CDC25A mRNA is stable during entire meiotic maturation. Exogenous CDC25A was sufficient to overcome cAMP-mediated GV-stage block. Using microinjection of GFP-CDC25A and GFP-CDC25B mRNAs constructs we have revealed that CDC25A is exclusively nuclear protein until nuclear envelope break down (NEBD). In contrast CDC25B localizes to cytoplasm at GV-stage oocytes and translocates to nucleus shortly before NEBD. Overexpression of GFP-CDC25A, to interfere with CDC25A degradation during meiotic maturation, resulted in MI block characterized with problems in chromosome congression and spindle formation. This MI block was accompanied with the transient reduction of both CDK1 and MAPK activities. RNAi mediated CDC25A knock-down resulted in a reduced ability to resume meiosis and to reach MII. These data demonstrate that behavior of CDC25A during female meiosis differs significantly from mitosis and CDC25A is involved in both, resumption of meiosis and also in metaphase I spindle formation as a prerequisite for correct MI-MII transition. It is evident that CDC25B is not only important CDC25 phosphatase for meiotic maturation but also CDC25A has its meiotic specific role. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2008
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