Plant Protection Science. - : Czech Academy of Agricultural Sciences
- ISSN 1212-2580
Roč. 41, - (2005), s. 52-57
Poč.str.
6 s.
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
Nicotiana tabacum ; PR-proteins ; PAGE
Vědní obor RIV
CE - Biochemie
CEP
GA522/02/0708 GA ČR - Grantová agentura ČR
CEZ
AV0Z50380511 - UEB-Q (2005-2011)
Anotace
The composition of pathogenesis-related proteins (PR-proteins) and inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15–16 kDa (Group 1), 27–28 kDa (Group 3: chitinases) and 36–40 kDa (Group 2a: β-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.
Pracoviště
Ústav experimentální botaniky
Kontakt
David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru
2006
Počet záznamů: 1
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