Počet záznamů: 1
Early onset of APC/C activity renders SAC inefficient in mouse embryos
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SYSNO ASEP 0584848 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Early onset of APC/C activity renders SAC inefficient in mouse embryos Tvůrce(i) Horáková, Adéla (UZFG-Y)
Konečná, Markéta (UZFG-Y)
Radoňová, Lenka (UZFG-Y)
Anger, Martin (UZFG-Y) ORCIDCelkový počet autorů 4 Číslo článku 1355979 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 12, Mar 13 (2024)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova spindle ; spindle assembly checkpoint ; chromosome segregation ; anaphase ; embryo ; Mad1 ; anaphase-promoting complex Obor OECD Reproductive biology (medical aspects to be 3) Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001190896200001 EID SCOPUS 85188590577 DOI 10.3389/fcell.2024.1355979 Anotace Control mechanisms of spindle assembly and chromosome segregation are vital for preventing aneuploidy during cell division. The mammalian germ cells and embryos are prone to chromosome segregation errors, and the resulting aneuploidy is a major cause of termination of development or severe developmental disorders. Here we focused on early mouse embryos, and using combination of methods involving microinjection, immunodetection and confocal live cell imaging, we concentrated on the Spindle Assembly Checkpoint (SAC) and Anaphase Promoting Complex/Cyclosome (APC/C). These are two important mechanisms cooperating during mitosis to ensure accurate chromosome segregation, and assessed their activity during the first two mitoses after fertilization. Our results showed, that in zygotes and 2-cell embryos, the SAC core protein Mad1 shows very low levels on kinetochores in comparison to oocytes and its interaction with chromosomes is restricted to a short time interval after nuclear membrane disassembly (NEBD). Exposure of 2-cell embryos to low levels of spindle poison does not prevent anaphase, despite the spindle damage induced by the drug. Lastly, the APC/C is activated coincidentally with NEBD before the spindle assembly completion. This early onset of APC/C activity, together with precocious relocalization of Mad1 from chromosomes, prevents proper surveillance of spindle assembly by SAC. The results contribute to the understanding of the origin of aneuploidy in early embryos. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2025 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2024.1355979/full
Počet záznamů: 1