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Synchrotron X-rays reveal the modes of Fe binding and trace metal storage in the brown algae Laminaria digitata and Ectocarpus siliculosus
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SYSNO ASEP 0583895 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Synchrotron X-rays reveal the modes of Fe binding and trace metal storage in the brown algae Laminaria digitata and Ectocarpus siliculosus Tvůrce(i) Mijovilovich, Ana (BC-A) ORCID, RID
Cloetens, P. (FR)
Lanzirotti, A. (US)
Newville, M. (US)
Wellenreuther, G. (DE)
Kumari, P. (GB)
Katsaros, C. (GR)
Carrano, C. J. (US)
Küpper, Hendrik (BC-A) RID, ORCID
Kupper, F. C. (GB)Celkový počet autorů 10 Číslo článku mfad058 Zdroj.dok. Metallomics. - : Oxford University Press - ISSN 1756-5901
Roč. 15, č. 10 (2023)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova algae ; iron ; strontium ; tomography ; xanes ; ferritin Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP EF15_003/0000336 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 001086526800001 EID SCOPUS 85176353578 DOI 10.1093/mtomcs/mfad058 Anotace Iron is accumulated symplastically in kelp in a non-ferritin core that seems to be a general feature of brown algae. Microprobe studies show that Fe binding depends on tissue type.The sea is generally an iron-poor environment and brown algae were recognized in recent years for having a unique, ferritin-free iron storage system. Kelp (Laminaria digitata) and the filamentous brown alga Ectocarpus siliculosus were investigated using X-ray microprobe imaging and nanoprobe X-ray fluorescence tomography to explore the localization of iron, arsenic, strontium, and zinc, and micro-X-ray absorption near-edge structure (mu XANES) to study Fe binding. Fe distribution in frozen hydrated environmental samples of both algae shows higher accumulation in the cortex with symplastic subcellular localization. This should be seen in the context of recent ultrastructural insight by cryofixation-freeze substitution that found a new type of cisternae that may have a storage function but differs from the apoplastic Fe accumulation found by conventional chemical fixation. Zn distribution co-localizes with Fe in E. siliculosus, whereas it is chiefly located in the L. digitata medulla, which is similar to As and Sr. Both As and Sr are mostly found at the cell wall of both algae. XANES spectra indicate that Fe in L. digitata is stored in a mineral non-ferritin core, due to the lack of ferritin-encoding genes. We show that the L. digitata cortex contains mostly a ferritin-like mineral, while the meristoderm may include an additional component. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://academic.oup.com/metallomics/article/15/10/mfad058/7281006?login=true
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