Počet záznamů: 1
Evaluation and optimization of an eDNA metabarcoding assay for detection of freshwater myxozoan communities
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SYSNO ASEP 0583120 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název Evaluation and optimization of an eDNA metabarcoding assay for detection of freshwater myxozoan communities Tvůrce(i) Lisnerová, Martina (BC-A) ORCID
Holzer, Astrid S. (BC-A) RID, ORCID
Blabolil, Petr (BC-A) RID, ORCID
Fiala, Ivan (BC-A) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. Environmental DNA
Roč. 5, č. 2 (2023), s. 312-325Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova comparative diversity ; environmental DNA ; eutrophic ; fish parasites ; phylogeny ; sediment Vědní obor RIV EH - Ekologie - společenstva Obor OECD Ecology CEP GX19-28399X GA ČR - Grantová agentura ČR QK1920011 GA MZe - Ministerstvo zemědělství Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 EID SCOPUS 85146095628 DOI 10.1002/edn3.380 Anotace The environmental DNA (eDNA) metabarcoding approach has become a useful tool for detecting the species diversity of different animal groups, including parasites. Myxozoa (Malacosporea and Myxosporea) represent a unique group of morphologically simplified endoparasites that mainly infest fish and whose diversity remains largely unexplored. Metabarcoding of DNA from the aquatic environment is a promising non-invasive method that allows us to assess myxozoan biodiversity at a given site. This is essential not only for describing myxozoan communities, but also for the development of disease control methods. Using an alignment of 330 sequences, we employed in silico PCR to score primer pairs, designed to amplify the V4 region of the SSU rDNA of different myxosporean sublineages comprising the entire diversity of oligochaete-infecting (freshwater) myxosporeans. We selected eight clade-specific primer sets for metabarcoding, avoiding amplification of DNA from other organisms present in eutrophic freshwaters. The metabarcoding approach used in the analysis of eDNA sediment samples detected a high myxosporean diversity even in small sample volumes (in total 44 OTUs). Furthermore, metabarcoding analysis of myxosporeans in fish tissue samples selected for primer testing revealed 91 different myxosporean OTUs, more than double the number obtained by classical PCR screening and Sanger sequencing with general myxozoan primers and almost seven times higher detection than by microscopic examination. Our results further suggest quantitative sampling requirements for realistic future diversity estimates by comparing OTUs from fish tissue metabarcoding and eDNA samples. The use of specific primer sets enabled the detection of a high proportion of myxosporean reads (63–100%) in all datasets, even in highly eutrophic habitats. This shows our metabarcoding approach as an excellent tool for non-invasive and sensitive detection of myxosporean biodiversity in aquatic sediments, potentially useful for monitoring myxozoan disease agents that threaten economically important fish in aquaculture. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1002/edn3.380
Počet záznamů: 1