Počet záznamů: 1
Differential gene expression and microRNA profile in icorpora allata-corpora cardiaca/i of iAedes aegypti/i mosquitoes with weak juvenile hormone signalling
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SYSNO ASEP 0582766 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differential gene expression and microRNA profile in icorpora allata-corpora cardiaca/i of iAedes aegypti/i mosquitoes with weak juvenile hormone signalling Tvůrce(i) Qi, Z. (US)
Etebari, K. (AU)
Nouzová, Marcela (BC-A) ORCID
Noriega, F. G. (US)
Asgari, S. (AU)Celkový počet autorů 5 Číslo článku 113 Zdroj.dok. BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 25, č. 1 (2024)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova troponin-t ; drosophila ; biosynthesis ; maintenance ; annotation ; evolution ; homolog ; pathway ; body ; Aedes aegypti ; Mosquito ; Transcriptome ; RNA-Seq ; Epoxidase ; Juvenile hormone ; microRNA ; Corpora allata Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA22-21244S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 001151282500001 EID SCOPUS 85183046576 DOI 10.1186/s12864-024-10007-9 Anotace The corpora allata-corpora cardiaca (CA-CC) is an endocrine gland complex that regulates mosquito development and reproduction through the synthesis of juvenile hormone (JH). Epoxidase (Epox) is a key enzyme in the production of JH. We recently utilized CRISPR/Cas9 to establish an epoxidase-deficient (epox(-/-)) Aedes aegypti line. The CA from epox(-/-) mutants do not synthesize epoxidated JH III but methyl farneosate (MF), a weak agonist of the JH receptor, and therefore have reduced JH signalling. Illumina sequencing was used to examine the differences in gene expression between the CA-CC from wild type (WT) and epox(-/-) adult female mosquitoes. From 18,034 identified genes, 317 were significantly differentially expressed. These genes are involved in many biological processes, including the regulation of cell proliferation and apoptosis, energy metabolism, and nutritional uptake. In addition, the same CA-CC samples were also used to examine the microRNA (miRNA) profiles of epox(-/- )and WT mosquitoes. A total of 197 miRNAs were detected, 24 of which were differentially regulated in epox(-/-) mutants. miRNA binding sites for these particular miRNAs were identified using an in silico approach, they target a total of 101 differentially expressed genes. Our results suggest that a lack of epoxidase, besides affecting JH synthesis, results in the diminishing of JH signalling that have significant effects on Ae. aegypti CA-CC transcriptome profiles, as well as its miRNA repertoire. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2025 Elektronická adresa https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10007-9
Počet záznamů: 1