Počet záznamů: 1
Mass Spectrometry-Based Proteomics
- 1.
SYSNO ASEP 0580418 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry Tvůrce(i) Kalaninová, Zuzana (MBU-M) ORCID, RID
Fojtík, Lukáš (MBU-M)
Chmelík, Josef (MBU-M) RID, ORCID
Novák, Petr (MBU-M) RID, ORCID
Volný, Michael (MBU-M) ORCID
Man, Petr (MBU-M) RID, ORCIDZdroj.dok. Mass Spectrometry-Based Proteomics. - New York : Humana, 2023 / Gevaert K. - ISSN 1064-3745 - ISBN 978-1-0716-3456-1 Rozsah stran s. 303-334 Poč.str. 32 s. Poč.str.knihy 336 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Antibody ; Hydrogen/deuterium exchange ; Mass spectrometry ; Proteolysis ; Protein structure and dynamics ; Biosimilars Obor OECD Biochemistry and molecular biology CEP GA22-27695S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 DOI 10.1007/978-1-0716-3457-8_17 Anotace Hydrogen/deuterium exchange (HDX) followed by mass spectrometry detection (MS) provides a fast, reliable, and detailed solution for the assessment of a protein structure. It has been widely recognized as an indispensable tool and already approved by several regulatory agencies as a structural technique for the validation of protein biopharmaceuticals, including antibody-based drugs. Antibodies are of a key importance in life and medical sciences but considered to be challenging analytical targets because of their compact structure stabilized by disulfide bonds and due to the presence of glycosylation. Despite these difficulties, there are already numerous excellent studies describing MS-based antibody structure characterization. In this chapter, we describe a universal HDX-MS workflow. Deeper attention is paid to sample handling, optimization procedures, and feasibility stages, as these elements of the HDX experiment are crucial for obtaining reliable detailed and spatially well-resolved information. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://link.springer.com/protocol/10.1007/978-1-0716-3457-8_17
Počet záznamů: 1