Počet záznamů: 1
Accumulation of cyanobacterial oxadiazine nocuolin A is enhanced by temperature shift during cultivation and is promoted by bacterial cohabitants in the culture
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SYSNO ASEP 0578785 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Accumulation of cyanobacterial oxadiazine nocuolin A is enhanced by temperature shift during cultivation and is promoted by bacterial cohabitants in the culture Tvůrce(i) Chmelík, D. (CZ)
Hrouzek, Pavel (MBU-M) ORCID
Fedorko, J. (CZ)
Vu, Dai Long (MBU-M) ORCID
Urajová, Petra (MBU-M)
Mareš, Jan (MBU-M) ORCID
Červený, J. (CZ)Číslo článku 101673 Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 44, DEC 2019 (2019)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova secondary metabolites ; growth ; nostocales ; Axenization ; Secondary metabolite ; Nocuolin A ; Cyanobacteria ; Biphasic cultivation Obor OECD Virology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000498792900001 EID SCOPUS 85075030455 DOI 10.1016/j.algal.2019.101673 Anotace Proper setting of cultivation conditions is essential for production of high-value compounds in microbial biotechnology. The present study characterizes photoautotrophic growth and capacity to accumulate the anti-proliferative secondary metabolite Nocuolin A (NoA) in cyanobacterium Nostoc sp. CCAP 1453/38. As the cyanobacterial culture was found to be non-axenic, the bacteria accompanying the culture were characterized, then the growth demands and NoA production in the Nostoc-bacterial consortium were determined, and finally an axenic strain was prepared. For the purposes of growth characterization, the culture was maintained in a quasi-continuous regime under various light intensities, temperatures, and inorganic carbon concentrations in a small-scale laboratory photobioreactor. The maximum biomass growth rate obtained was 0.10 h(-1) (doubling time Dt = 6.93 h). Following optimal growth conditions were identified: temperature of 35 degrees C, light intensity 600 mu mol(photons) M-2 s(-1), and 2500 ppm CO2 in the sparging gas. As the temperature optima for the biomass production and for NoA accumulation differed, biphasic cultivation for maximal NoA yield was designed, leading to a three times more effective cultivation procedure compared to batch culture maintained at a temperature optimal for NoA production. The increased NoA accumulation at reduced temperature that correlated with enhanced expression of NoA biosynthetic genes after the temperature shift suggested its regulation occurs at the expression level. It has further been shown that NoA production is reduced in axenic culture, which indicates that it is also triggered by presence of bacteria. This study shows an example of how a biphasic cultivation mode with different temperatures can be used in high-value compound production processes. It also brings direct evidence that cyanobacterial strain axenization can lead to a rapid decrease in production of valuable compounds and that non-axenic strains may be considered more suitable for retrieval and initial production of novel pharmaceutical leads. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2211926419302061?via%3Dihub
Počet záznamů: 1