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Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy: studies with pancreatic adenocarcinoma cells in vitro and in vivo
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SYSNO ASEP 0578782 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy: studies with pancreatic adenocarcinoma cells in vitro and in vivo Tvůrce(i) Shapoval, Oleksandr (UMCH-V) ORCID, RID
Větvička, D. (CZ)
Patsula, Vitalii (UMCH-V) RID, ORCID
Engstová, Hana (FGU-C) RID, ORCID
Kočková, Olga (UMCH-V) RID, ORCID
Konefal, Magdalena (UMCH-V) ORCID, RID
Kabešová, M. (CZ)
Horák, Daniel (UMCH-V) RID, ORCIDČíslo článku 2694 Zdroj.dok. Pharmaceutics. - : MDPI
Roč. 15, č. 12 (2023)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova upconversion ; temoporfin ; photodynamic therapy Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-materials (production and properties) Vědní obor RIV – spolupráce Fyziologický ústav - Morfologické obory a cytologie CEP LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 001130799500001 EID SCOPUS 85180642892 DOI 10.3390/pharmaceutics15122694 Anotace Upconverting nanoparticles are interesting materials that have the potential for use in many applications ranging from solar energy harvesting to biosensing, light-triggered drug delivery, and photodynamic therapy (PDT). One of the main requirements for the particles is their surface modification, in our case using poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and temoporfin (THPC) photosensitizer to ensure the colloidal and chemical stability of the particles in aqueous media and the formation of singlet oxygen after NIR irradiation, respectively. Codoping of Fe2+, Yb3+, and Er3+ ions in the NaYF4 host induced upconversion emission of particles in the red region, which is dominant for achieving direct excitation of THPC. Novel monodisperse PMVEMA-coated upconversion NaYF4:Yb3+,Er3+,Fe2+ nanoparticles (UCNPs) with chemically bonded THPC were found to efficiently transfer energy and generate singlet oxygen. The cytotoxicity of the UCNPs was determined in the human pancreatic adenocarcinoma cell lines Capan-2, PANC-01, and PA-TU-8902. In vitro data demonstrated enhanced uptake of UCNP@PMVEMA-THPC particles by rat INS-1E insulinoma cells, followed by significant cell destruction after excitation with a 980 nm laser. Intratumoral administration of these nanoconjugates into a mouse model of human pancreatic adenocarcinoma caused extensive necrosis at the tumor site, followed by tumor suppression after NIR-induced PDT. In vitro and in vivo results thus suggest that this nanoconjugate is a promising candidate for NIR-induced PDT of cancer. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://www.mdpi.com/1999-4923/15/12/2694
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