Počet záznamů: 1  

Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy: studies with pancreatic adenocarcinoma cells in vitro and in vivo

  1. 1.
    SYSNO ASEP0578782
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevTemoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy: studies with pancreatic adenocarcinoma cells in vitro and in vivo
    Tvůrce(i) Shapoval, Oleksandr (UMCH-V) ORCID, RID
    Větvička, D. (CZ)
    Patsula, Vitalii (UMCH-V) RID, ORCID
    Engstová, Hana (FGU-C) RID, ORCID
    Kočková, Olga (UMCH-V) RID, ORCID
    Konefal, Magdalena (UMCH-V) ORCID, RID
    Kabešová, M. (CZ)
    Horák, Daniel (UMCH-V) RID, ORCID
    Číslo článku2694
    Zdroj.dok.Pharmaceutics. - : MDPI
    Roč. 15, č. 12 (2023)
    Poč.str.18 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovaupconversion ; temoporfin ; photodynamic therapy
    Vědní obor RIVJJ - Ostatní materiály
    Obor OECDNano-materials (production and properties)
    Vědní obor RIV – spolupráceFyziologický ústav - Morfologické obory a cytologie
    CEPLX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaUMCH-V - RVO:61389013 ; FGU-C - RVO:67985823
    UT WOS001130799500001
    EID SCOPUS85180642892
    DOI10.3390/pharmaceutics15122694
    AnotaceUpconverting nanoparticles are interesting materials that have the potential for use in many applications ranging from solar energy harvesting to biosensing, light-triggered drug delivery, and photodynamic therapy (PDT). One of the main requirements for the particles is their surface modification, in our case using poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and temoporfin (THPC) photosensitizer to ensure the colloidal and chemical stability of the particles in aqueous media and the formation of singlet oxygen after NIR irradiation, respectively. Codoping of Fe2+, Yb3+, and Er3+ ions in the NaYF4 host induced upconversion emission of particles in the red region, which is dominant for achieving direct excitation of THPC. Novel monodisperse PMVEMA-coated upconversion NaYF4:Yb3+,Er3+,Fe2+ nanoparticles (UCNPs) with chemically bonded THPC were found to efficiently transfer energy and generate singlet oxygen. The cytotoxicity of the UCNPs was determined in the human pancreatic adenocarcinoma cell lines Capan-2, PANC-01, and PA-TU-8902. In vitro data demonstrated enhanced uptake of UCNP@PMVEMA-THPC particles by rat INS-1E insulinoma cells, followed by significant cell destruction after excitation with a 980 nm laser. Intratumoral administration of these nanoconjugates into a mouse model of human pancreatic adenocarcinoma caused extensive necrosis at the tumor site, followed by tumor suppression after NIR-induced PDT. In vitro and in vivo results thus suggest that this nanoconjugate is a promising candidate for NIR-induced PDT of cancer.
    PracovištěÚstav makromolekulární chemie
    KontaktEva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
    Rok sběru2024
    Elektronická adresahttps://www.mdpi.com/1999-4923/15/12/2694
Počet záznamů: 1  

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