Počet záznamů: 1
Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis
- 1.
SYSNO ASEP 0577394 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis Tvůrce(i) Siems, K. (DE)
Runzheimer, K. (DE)
Rebrošová, K. (CZ)
Etzbach, L. (DE)
Auerhammer, A. (DE)
Rehm, A. (DE)
Schwengers, O. (DE)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Růžička, F. (CZ)
Moeller, R. (DE)Celkový počet autorů 11 Číslo článku 1272734 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 14, 29 September (2023)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Staphylococcus capitis ; staphyloxanthin ; coagulase-negative staphylococci (CoNS) ; carotenoids ; bacterial pigments Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP NU21-05-00341 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora UPT-D - RVO:68081731 UT WOS 001085828700001 EID SCOPUS 85174573280 DOI 10.3389/fmicb.2023.1272734 Anotace Introduction Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity.Methods In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress.Results We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (-20 degrees C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains.Conclusion The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2024 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full
Počet záznamů: 1