Počet záznamů: 1
Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella
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SYSNO ASEP 0574095 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella Tvůrce(i) Pospíšilová, Kristýna (BC-A) ORCID
Van ’t Hof, Arjen (BC-A)
Yoshido, Atsuo (BC-A) RID, ORCID
Kružíková, Renata (BC-A)
Visser, Sander (BC-A) ORCID
Zrzavá, Magda (BC-A) RID, ORCID
Bobryshava, K. (CZ)
Dalíková, M. (CZ)
Marec, František (BC-A) RID, ORCIDCelkový počet autorů 9 Číslo článku 103991 Zdroj.dok. Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748
Roč. 160, SEP 01 (2023)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova sex determination ; Masculinizer ; Lepidoptera Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GA20-13784S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 001053153200001 EID SCOPUS 85166643232 DOI 10.1016/j.ibmb.2023.103991 Anotace The molecular mechanisms of sex determination in moths and butterflies (Lepidoptera) with female heterogamety (WZ/ZZ) are poorly understood, except in the silkworm Bombyx mori. However, the Masculinizer (Masc) gene that controls male development and dosage compensation in B. mori, appears to be conserved in Lepidoptera, as its masculinizing function was recently confirmed in several moth species. In this work, we investigated the role of the Masc gene in sex determination of the codling moth Cydia pomonella (Tortricidae), a globally important pest of pome fruits and walnuts. The gene structure of the C. pomonella Masc ortholog, CpMasc, is similar to B. mori Masc. However, unlike B. mori, we identified 14 splice variants of CpMasc in the available transcriptomes. Subsequent screening for sex specificity and genetic variation using publicly available data and RT-PCR revealed three male-specific splice variants. Then qPCR analysis of these variants revealed sex-biased expression showing a peak only in early male embryos. Knockdown of CpMasc by RNAi during early embryogenesis resulted in a shift from male-to female-specific splicing of the C. pomonella doublesex (Cpdsx) gene, its downstream effector, in ZZ embryos, leading to a strongly female-biased sex ratio. These data clearly demonstrate that CpMasc functions as a masculinizing gene in the sex-determining cascade of C. pomonella. Our study also showed that CpMasc transcripts are provided maternally, as they were detected in unfertilized eggs after oviposition and in mature eggs dissected from virgin females. This finding is unique, as maternal provision of mRNA has rarely been studied in Lepidoptera. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0965174823000851/pdfft?md5=70a9a32dc6a58aacc943d40ee7d3fb8b&pid=1-s2.0-S0965174823000851-main.pdf
Počet záznamů: 1