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Spontaneous Regression of Swine Melanoma: The Role of Tumour-infiltrating T and NK Cells
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SYSNO ASEP 0573867 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Spontaneous Regression of Swine Melanoma: The Role of Tumour-infiltrating T and NK Cells Tvůrce(i) Plánská, Daniela (UZFG-Y)
Horák, Vratislav (UZFG-Y) RID, ORCIDZdroj.dok. Anticancer Research. - : International Institute of Anticancer Research - ISSN 0250-7005
Roč. 43, č. 2 (2023), s. 631-638Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GR - Řecko Klíč. slova melanoma ; spontaneous regression ; Libechov Minipigs Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Oncology CEP EF16_019/0000785 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001013271000007 EID SCOPUS 85146879104 DOI 10.21873/anticanres.16199 Anotace Background/Aim: Melanoma is a skin cancer without effective therapy, showing high immunogenicity and mostly partial spontaneous regression (SR). The exact mechanisms of SR are still not well understood, therefore, the use of animal melanoma models is necessary to unravel the immunological processes during SR. Materials and Methods: Skin melanoma samples (n=57) and peripheral blood samples (n=57) from the same animals were collected. Melanoma -bearing Libechov Minipigs (MeLiM) aged 3, 4, 6, 8, 10, 12, 20, and 32 weeks were used, and samples were analysed by flow cytometry for detection of immune cell subpopulations. Results: The proportion of CD3-CD8+ (NK) cells in melanoma samples was found to be higher compared to blood samples at 6-8 weeks of age and then at 12 weeks of age. The population of CD4+CD8+ (effector/memory T helper) cells and CD4-CD8+ (cytotoxic T and NK) cells was also increased in melanoma compared to blood samples in 10-32 -week-old pigs. The proportion of CD4-CD8+ cells in melanoma samples, then augmented until the 32nd week. On the contrary, the proportion of CD4+CD8- (naive T helper) cells was lower in melanoma samples versus blood samples in 6-32-week-old animals. Conclusion: Cytotoxic T cells were the most abundant population of tumour infiltrating immune cells found in MeLiM melanomas of animals aged 10-32 weeks, probably causing the destruction of melanoma cells. Furthermore, the development of specific (adaptive) immune response represented mainly by cytotoxic T cells seems to be crucial for the successful SR of porcine melanoma. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2024 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=54086803247
Počet záznamů: 1