Počet záznamů: 1
Recent Progress in the Production of Cyanide-Converting Nitrilases-Comparison with Nitrile-Hydrolyzing Enzymes
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SYSNO ASEP 0571188 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Recent Progress in the Production of Cyanide-Converting Nitrilases-Comparison with Nitrile-Hydrolyzing Enzymes Tvůrce(i) Martínková, Ludmila (MBU-M) RID, ORCID
Kulik, Natalia (MBU-M) ORCID
Sedova, A. (CZ)
Křístková, Barbora (MBU-M)
Bojarová, Pavla (MBU-M) ORCIDČíslo článku 500 Zdroj.dok. Catalysts. - : MDPI
Roč. 13, č. 3 (2023)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova cyanide dihydratase ; cyanide hydratase ; cyanide removal ; cyanide sensor ; enzyme purification ; immobilization ; nitrilase ; overexpression ; phylogenetic analysis ; whole-cell catalyst Obor OECD Environmental biotechnology CEP GA22-06785S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000954124300001 EID SCOPUS 85151534333 DOI 10.3390/catal13030500 Anotace Nitrilases have a high potential for application in organic chemistry, environmental technology, and analytics. However, their industrial uses require that they are produced in highly active and robust forms at a reasonable cost. Some organic syntheses catalyzed by nitrilases have already reached a high level of technological readiness. This has been enabled by the large-scale production of recombinant catalysts. Despite some promising small-scale methods being proposed, the production of cyanide-converting nitrilases (cyanide hydratase and cyanide dihydratase) is lagging in this regard. This review focuses on the prospects of cyanide(di)hydratase-based catalysts. The current knowledge of these enzymes is summarized and discussed in terms of the origin and distribution of their sequences, gene expression, structure, assays, purification, immobilization, and uses. Progresses in the production of other nitrilase catalysts are also tackled, as it may inspire the development of the preparation processes of cyanide(di)hydratases. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://www.mdpi.com/2073-4344/13/3/500
Počet záznamů: 1