Počet záznamů: 1
The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAir (TM))
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SYSNO ASEP 0567681 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAir (TM)) Tvůrce(i) Šíma, Michal (UEM-P) RID, ORCID
Červená, Tereza (UEM-P) ORCID, RID
Elzeinová, Fatima (UEM-P)
Ambrož, Antonín (UEM-P)
Beránek, V. (CZ)
Vojtíšek-Lom, M. (CZ)
Cigánek, M. (CZ)
Rössner ml., Pavel (UEM-P) RID, ORCIDČíslo článku 105316 Zdroj.dok. Toxicology in Vitro. - : Elsevier - ISSN 0887-2333
Roč. 80, apr (2022)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova BEAS-2B ; mucilAir (TM) ; extractable organic matter ; exposure ; ethanol blend fuels Obor OECD Public and environmental health CEP GA18-04719S GA ČR - Grantová agentura ČR LM2018124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018133 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001821 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000777999500003 EID SCOPUS 85123242016 DOI 10.1016/j.tiv.2022.105316 Anotace Air pollution caused by road traffic has an unfavorable impact on the environment and also on human health. It has previously been shown, that complete gasoline emissions lead to toxic effects in cell models originating from human airways. Here we focused on extractable organic matter (EOM) from particulate matter, collected from gasoline emissions from fuels with different ethanol content. We performed cytotoxicity evaluation, quantification of mucin and extracellular reactive oxygen species (ROS) production, DNA breaks detection, and selected gene deregulation analysis, after one and five days of exposure of human bronchial epithelial model (BEAS-2B) and a 3D model of the human airway (MucilAir (TM)). Our data suggest that the longer exposure had more pronounced effects on the parameters of cytotoxicity and mucin production, while the impacts on ROS generation and DNA integrity were limited. In both cell models the expression of CYP1A1 was induced, regardless of the exposure period or EOM tested. Several other genes, including FMO2, IL1A, or TNF, were deregulated depending on the exposure time. In conclusion, ethanol content in the fuels did not significantly impact the toxicity of EOM. Biological effects were mostly linked to xenobiotics metabolism and inflammatory response. BEAS-2B cells were more sensitive to the treatment. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0887233322000133?via%3Dihub
Počet záznamů: 1