Počet záznamů: 1
Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats
- 1.
SYSNO ASEP 0565357 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats Tvůrce(i) Berková, Z. (CZ)
Zacharovová, K. (CZ)
Pátiková, A. (CZ)
Leontovyc, I. (CZ)
Hladíková, Z. (CZ)
Červený, D. (CZ)
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Girman, P. (CZ)
Jirák, D. (CZ)
Saudek, F. (CZ)Číslo článku 171 Zdroj.dok. Journal of Functional Biomaterials. - : MDPI - ISSN 2079-4983
Roč. 13, č. 4 (2022)Poč.str. 17 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova pancreas decellularization ; splenic vein perfusion ; extracellular matrix skeletons ; transplantation into the omentum ; advanced environmental scanning electron microscopy Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP GA22-25799S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UPT-D - RVO:68081731 UT WOS 000901294600001 EID SCOPUS 85144872942 DOI 10.3390/jfb13040171 Anotace Infusing pancreatic islets into the portal vein currently represents the preferred approach for islet transplantation, despite considerable loss of islet mass almost immediately after implantation. Therefore, approaches that obviate direct intravascular placement are urgently needed. A promising candidate for extrahepatic placement is the omentum. We aimed to develop an extracellular matrix skeleton from the native pancreas that could provide a microenvironment for islet survival in an omental flap. To that end, we compared different decellularization approaches, including perfusion through the pancreatic duct, gastric artery, portal vein, and a novel method through the splenic vein. Decellularized skeletons were compared for size, residual DNA content, protein composition, histology, electron microscopy, and MR imaging after repopulation with isolated islets. Compared to the other approaches, pancreatic perfusion via the splenic vein provided smaller extracellular matrix skeletons, which facilitated transplantation into the omentum, without compromising other requirements, such as the complete depletion of cellular components and the preservation of pancreatic extracellular proteins. Repeated MR imaging of iron-oxide-labeled pancreatic islets showed that islets maintained their position in vivo for 49 days. Advanced environmental scanning electron microscopy demonstrated that islets remained integrated with the pancreatic skeleton. This novel approach represents a proof-of-concept for long-term transplantation experiments. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/2079-4983/13/4/171
Počet záznamů: 1